Validation of high throughput sequencing and microbial forensics applications

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dc.contributor.authorBudowle, Bruce
dc.contributor.authorConnell, Nancy D
dc.contributor.authorBielecka-Oder, Anna
dc.contributor.authorColwell, Rita R
dc.contributor.authorCorbett, Cindi R
dc.contributor.authorFletcher, Jacqueline
dc.contributor.authorForsman, Mats
dc.contributor.authorKadavy, Dana R
dc.contributor.authorMarkotic, Alemka
dc.contributor.authorMorse, Stephen A
dc.contributor.authorMurch, Randall S
dc.contributor.authorSajantila, Antti
dc.contributor.authorSchmedes, Sarah E
dc.contributor.authorTernus, Krista L
dc.contributor.authorTurner, Stephen D
dc.contributor.authorMinot, Samuel
dc.date.accessioned2014-08-06T19:06:09Z
dc.date.available2014-08-06T19:06:09Z
dc.date.issued2014-07-30
dc.date.updated2014-08-06T19:06:12Z
dc.description.abstractAbstract High throughput sequencing (HTS) generates large amounts of high quality sequence data for microbial genomics. The value of HTS for microbial forensics is the speed at which evidence can be collected and the power to characterize microbial-related evidence to solve biocrimes and bioterrorist events. As HTS technologies continue to improve, they provide increasingly powerful sets of tools to support the entire field of microbial forensics. Accurate, credible results allow analysis and interpretation, significantly influencing the course and/or focus of an investigation, and can impact the response of the government to an attack having individual, political, economic or military consequences. Interpretation of the results of microbial forensic analyses relies on understanding the performance and limitations of HTS methods, including analytical processes, assays and data interpretation. The utility of HTS must be defined carefully within established operating conditions and tolerances. Validation is essential in the development and implementation of microbial forensics methods used for formulating investigative leads attribution. HTS strategies vary, requiring guiding principles for HTS system validation. Three initial aspects of HTS, irrespective of chemistry, instrumentation or software are: 1) sample preparation, 2) sequencing, and 3) data analysis. Criteria that should be considered for HTS validation for microbial forensics are presented here. Validation should be defined in terms of specific application and the criteria described here comprise a foundation for investigators to establish, validate and implement HTS as a tool in microbial forensics, enhancing public safety and national security.
dc.description.versionPeer Reviewed
dc.identifier.citationInvestigative Genetics. 2014 Jul 30;5(1):9
dc.identifier.doihttp://dx.doi.org/10.1186/2041-2223-5-9
dc.identifier.urihttp://hdl.handle.net/1993/23742
dc.language.rfc3066en
dc.rightsopen accessen_US
dc.rights.holderBruce Budowle et al.; licensee BioMed Central Ltd.
dc.titleValidation of high throughput sequencing and microbial forensics applications
dc.typeJournal Article
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