Cloning and characterization of novel IgA antibody variable heavy and light chains from HIV-1 resistant sex workers from Nairobi, Kenya

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dc.contributor.authorSarna, Caitlin S.
dc.contributor.examiningcommitteeFowke, Keith R. (Medical Microbiology) Berry, Jody D. (Immunology)en
dc.contributor.supervisorPlummer, Francis A. (Medical Microbiology)en
dc.date.accessioned2011-04-14T19:54:46Z
dc.date.available2011-04-14T19:54:46Z
dc.date.issued2011-04-14T19:54:46Z
dc.degree.disciplineMedical Microbiologyen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractHeterosexual intercourse now accounts for the majority of HIV transmission within sub-Saharan Africa. The generation of microbicides and vaccines, therefore, requires a better understanding of the mucosal correlates of protection, including the role of HIV-specific IgA. It is now accepted that not all individuals are equally susceptible to HIV-1 infection, as exemplified by the HIV Exposed Seronegative (HESN) women of the Pumwani Cohort in Nairobi, Kenya. To assess whether mucosal IgA responses contribute to this protection, 3 novel IgA variable genes were cloned from HESN cervical B-cell cDNA. Nine monoclonal IgA Abs were produced, two of which were properly produced from cell culture. The HESN-derived A6/30L and A9/30L variants had a greater specificity for gp120IIIB than their A6/4L and A9/4L counterparts, while the A6 variant recognizes a distinct gp120 epitope compared to the broadly neutralizing antibody IgGb12. Further characterization of these IgA chains may suggest their suitability for use in microbicides or mucosal vaccines.en
dc.description.noteMay 2011en
dc.format.extent1237514 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/1993/4589
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectIgAen
dc.subjectHIV-1en
dc.subjectgp120en
dc.subjectMonoclonal antibodiesen
dc.subjectHESNen
dc.subjectMucosal immunologyen
dc.titleCloning and characterization of novel IgA antibody variable heavy and light chains from HIV-1 resistant sex workers from Nairobi, Kenyaen
dc.typemaster thesisen_US
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