Molecular characterization of ciprofloxacin resistant and/or beta-lactamase producing Escherichia coli from the Vancouver Coastal Health region

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dc.contributor.authorGonsalves, Elizabeth A.
dc.contributor.examiningcommitteeGraham, Morag (Medical Microbiology) Wylie John (Medical Microbiology) Worobec, Elizabeth (Microbiology)en_US
dc.contributor.supervisorMulvey, Michael (Medical Microbiology)en_US
dc.date.accessioned2011-09-12T14:34:59Z
dc.date.available2011-09-12T14:34:59Z
dc.date.issued2011-09-12
dc.degree.disciplineMedical Microbiologyen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractEmergent multidrug resistant Escherichia coli increase clinical challenges. This thesis describes the resistance patterns, molecular epidemiology and mechanisms, for 315 E. coli from patients in the Vancouver Coastal Health Region for 2006/2007. Automated susceptibility testing was confirmed via E-test® for AmpC and/or ESBL production. PFGE, RFLP and PCR were used to assess genotypic relationships, and plasmid character. AmpC production was facilitated mainly by promoter mutations (54.5%). The principal ESBL detected was CTX-M-15 (49.5%). An unidentified ESBL-producer, with a pI near 8.3, was detected. A plasmid displayed variant resistance phenotypes dependent on selective growth media. A positive correlation between ST131 with CTX-M-15 and CIPR indicated the dissemination of companion phenotypes. Ciprofloxacin resistance resulted mainly (98.0%) from a double gyrA mutation. Overall fluoroquinolone resistance was not assessable due to exclusive selection parameters in this evaluation. Fluoroquinolone resistance factors require further examination to understand what causes resistant phenotypes exclusive of chromosomal mutations. iien_US
dc.description.noteOctober 2011en_US
dc.identifier.urihttp://hdl.handle.net/1993/4886
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectepidemiologyen_US
dc.subjectresistanceen_US
dc.subjectfluoroquinoloneen_US
dc.subjectciprofloxacinen_US
dc.subjectbeta-lactamaseen_US
dc.subjectESBLen_US
dc.subjectE. colien_US
dc.subjectAmpCen_US
dc.titleMolecular characterization of ciprofloxacin resistant and/or beta-lactamase producing Escherichia coli from the Vancouver Coastal Health regionen_US
dc.typemaster thesisen_US
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