dc.contributor.author	Schmälter, Ann-Kristin
dc.contributor.author	Kuzyk, Alexandra
dc.contributor.author	Righolt, Christiaan H
dc.contributor.author	Neusser, Michaela
dc.contributor.author	Steinlein, Ortrud K
dc.contributor.author	Müller, Stefan
dc.contributor.author	Mai, Sabine
dc.date.accessioned	2014-07-02T15:05:36Z
dc.date.available	2014-07-02T15:05:36Z
dc.date.issued	2014-06-12
dc.date.updated	2014-07-02T15:05:36Z
dc.description.abstract	Abstract Background Characterizing the nuclear orientation of chromosomes in the three-dimensional (3D) nucleus by multicolor banding (mBANDing) is a new approach towards understanding nuclear organization of chromosome territories. An mBANDing paint is composed of multiple overlapping subchromosomal probes that represent different regions of a single chromosome. In this study, we used it for the analysis of chromosome orientation in 3D interphase nuclei. We determined whether the nuclear orientation of the two chromosome 11 homologs was random or preferential, and if it was conserved between diploid mouse Pre B lymphocytes of BALB/c origin and primary B lymphocytes of congenic [T38HxBALB/c]N wild-type mice. The chromosome orientation was assessed visually and through a semi-automated quantitative analysis of the radial and angular orientation patterns observed in both B cell types. Results Our data indicate that there are different preferential patterns of chromosome 11 orientation, which are not significantly different between both mouse cell types (p > 0.05). In the most common case for both cell types, both copies of chromosome 11 were oriented in parallel with the nuclear border. The second most common pattern in both types of B lymphocytes was with one homolog of chromosome 11 positioned with its telomeric end towards the nuclear center and with its centromeric end towards the periphery, while the other chromosome 11 was found parallel with the nuclear border. In addition to these two most common orientations present in approximately 50% of nuclei from each cell type, other orientations were observed at lower frequencies. Conclusions We conclude that there are probabilistic, non-random orientation patterns for mouse chromosome 11 in the mouse B lymphocytes we investigated (p < 0.0001).
dc.description.version	Peer Reviewed
dc.identifier.citation	BMC Cell Biology. 2014 Jun 12;15(1):22
dc.identifier.doi	http://dx.doi.org/10.1186/1471-2121-15-22
dc.identifier.uri	http://hdl.handle.net/1993/23675
dc.language.rfc3066	en
dc.rights	open access	en_US
dc.rights.holder	Ann-Kristin Schmälter et al.; licensee BioMed Central Ltd.
dc.title	Distinct nuclear orientation patterns for mouse chromosome 11 in normal B lymphocytes
dc.type	Journal Article

Distinct nuclear orientation patterns for mouse chromosome 11 in normal B lymphocytes

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