Development and Characterization of Magnetic Nanoparticles for Treating Glioblastoma

Simple item page
dc.contributor.authorKim, Suhyun
dc.date.accessioned2018-10-02T20:00:00Z
dc.date.available2018-10-02T20:00:00Z
dc.date.issued2018-08-23
dc.date.submitted2018-10-02T20:00:00Zen
dc.description.abstractGlioblastoma multiforme (GB) is the most common and most aggressive brain cancer in adults with a median survival time after diagnosis of 12 months even with multimodal treatments. Chemotherapeutics have limited response in GB due to inadequate drug accumulation at the tumor site. In response to limited chemotherapeutic options for treating GB, many researchers are investigating the use of nanoparticles (NPs), synthetically engineered particulates that are < 100 nm in size, as a novel drug delivery mechanism. The present study examined two different non-spherical magnetic nanoparticles in an effort to understand the mechanisms governing nanoparticle accumulation, cellular drug delivery and cytotoxicity in established human GB cell lines. The hypothesis of these studies is that nanoparticle accumulation occurs through specific endocytic pathways that can be altered by application of external magnetic fields. Two non-spherical shaped iron oxide nanoparticles (IONPs), iron oxide nanodiscs (IONDs) and iron oxide nanobricks (IONBs) with identical surface coating of N-(trimethoxysilylpropyl) ethylenediaminetriacetate trisodium salt (EDT) were evaluated for cellular accumulation, drug delivery and cytotoxic response profiles using established human GB cell lines. Both IONP compositions showed both temperature-dependent and magnetic field-dependent cellular accumulation. Cell accumulation appeared to be through a caveolin-based endocytic pathway. Doxorubicin (DOX) loading onto the IONP was greatest for EDT-IONB composition and these nanoparticles were further shown to produce cytoxic responses in GB cells. Based on these studies, EDT-IONB is a reasonable nanoparticle composition to advance for further in vivo studies for glioblastoma treatment.en_US
dc.description.sponsorshipNatural Science and Engineering Research Council of Canada and Canadian Institutes of Health Research.en_US
dc.identifier.citationAMAen_US
dc.identifier.urihttp://hdl.handle.net/1993/33504
dc.rightsopen accessen_US
dc.subjectGlioblastoma multiforme (GB)en_US
dc.subjectEDT-IONBen_US
dc.subjectnanoparticles (NPs)en_US
dc.subjectiron oxide nanoparticles (IONPs)en_US
dc.titleDevelopment and Characterization of Magnetic Nanoparticles for Treating Glioblastomaen_US
dc.typebachelor thesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Kim_BSc(Med) Report.pdf
Size:
2.27 MB
Format:
Adobe Portable Document Format
Description:
BSc(Med) Final Research Report
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed to upon submission
Description:
Download
Collections
College of Medicine - B.Sc. (Med) Projects