Street LAMP: loop-mediated isothermal amplification testing as an easily modifiable point of care rapid testing platform in at-risk populations
| dc.contributor.author | Barber-Dueck, Owen | |
| dc.contributor.supervisor | Keynan, Yoav | |
| dc.contributor.supervisor | MacKenzie, Lauren | |
| dc.date.accessioned | 2023-11-21T21:05:38Z | |
| dc.date.available | 2023-11-21T21:05:38Z | |
| dc.date.issued | 2022-08-03 | |
| dc.date.submitted | 2023-11-21T21:05:38Z | en_US |
| dc.degree.discipline | Medicine | en_US |
| dc.degree.level | Bachelor of Science in Medicine (B.Sc.Med.) | |
| dc.description.abstract | The COVID-19 pandemic has shifted rapid testing for infectious disease into a cultural spotlight, and interest and demand for rapid testing has subsequently increased as a result. The ability to confirm diagnoses at point of care and immediately deploy contact tracing, prevention, and treatment has proven advantages, especially in at-risk and vulnerable communities. This report investigates loop-mediated isothermal amplification (LAMP) assays as a platform for rapid testing with the intention of using it at homeless shelters and drop-ins for unsheltered people. This platform would allow community-based testing programs to test individuals cheaply, rapidly, and accurately. By using a single reaction mixture and swapping in or out primers for different pathogens, these testing programs allow community-informed testing. We validated reverse transcriptase LAMP (RT-LAMP) with previously RT-PCR tested SARS-CoV-2 swab samples (N=200) and, by using primers for multiple gene targets, determined a sensitivity of 75.00% (95% CI 65.34-83.12%), and a specificity of 98.99% (95% CI 94.50-99.97%). Additionally, we determined a sensitivity of 95.24% (95% CI 68-99%) and a specificity of 100% (95% CI 80-100%) for the use of LAMP testing in swabs of syphilis lesions (N=40). Finally, Bartonella quintana pooled lice samples (N=27) showed a sensitivity of 76% (95% CI 50-93%) and a specificity of 100% (95% CI 69-100%). These early findings and the ability to quickly respond to population specific needs with a modular, modifiable testing platforms merit further validation of these tests in real-world conditions, where they could be assessed for ease of use, cost-benefit, patient acceptability, and health outcomes. | en_US |
| dc.identifier.uri | http://hdl.handle.net/1993/37804 | |
| dc.language.iso | eng | |
| dc.rights | open access | en_US |
| dc.subject | Street LAMP | |
| dc.title | Street LAMP: loop-mediated isothermal amplification testing as an easily modifiable point of care rapid testing platform in at-risk populations | |
| dc.type | bachelor thesis | en_US |
| local.author.affiliation | Rady Faculty of Health Sciences::Max Rady College of Medicine::Department of Medical Microbiology and Infectious Diseases |