Role of myeloid-derived suppressor cells in TNBS-induced murine colitis

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dc.contributor.authorMoreno Martinez, Sem
dc.contributor.examiningcommitteeKung, Sam (Immunology) Warrington, Richard (Internal Medicine)en_US
dc.contributor.supervisorPeng, Zhikang (Immunology)en_US
dc.date.accessioned2012-10-25T14:49:00Z
dc.date.available2012-10-25T14:49:00Z
dc.date.issued2012-10-25
dc.degree.disciplineImmunologyen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractMyeloid-derived suppressor cells (MDSCs), characterized by the co-expression of CD11b and Gr1, are a heterogeneous population of immature myeloid cells that exhibit strong suppressive functions against T cell responses. In inflammatory conditions like IBD, there is an increase in MDSCs but this is not sufficient to improve intestinal inflammation in IBD. Herein, we investigated the expansion of MDSCs in TNBS-induced acute colitis and whether the adoptive transfer of in vitro generated MDSCs ameliorated intestinal inflammation. We found that CD11b+Gr1+ MDSCs were significantly increased in experimental colitis. Further, this increase correlated to some extent with the severity of the disease. As per our protocol, MDSCs were generated from bone marrow cells co-cultured with hepatic stellate cells (HSCs), an essential cell type to obtain functional MDSCs in vitro. Adoptive transfer of HSC-induced MDSCs improved body weight loss and significantly downregulated inflammatory cytokines TNF, IFN-γ, and IL-17 in colonic tissue. Our results indicate MDSCs are immunoregulatory players in intestinal inflammation and that the adoptive transfer of in vitro generated MDSCs may provide a novel therapeutic approach for inflammatory bowel disease.en_US
dc.description.noteFebruary 2013en_US
dc.identifier.urihttp://hdl.handle.net/1993/9676
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectMyeloid-derived suppressor cellsen_US
dc.subjectInflammatory bowel diseaseen_US
dc.subjectImmunologyen_US
dc.titleRole of myeloid-derived suppressor cells in TNBS-induced murine colitisen_US
dc.typemaster thesisen_US
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