Show simple item record

dc.contributor.supervisor Dhalla, Naranjan (Physiology) en_US
dc.contributor.author Muller, Alison L.
dc.date.accessioned 2012-08-28T20:05:53Z
dc.date.available 2012-08-28T20:05:53Z
dc.date.issued 2012-08-28
dc.identifier.uri http://hdl.handle.net/1993/8582
dc.description.abstract Previous studies have shown that ischemia-reperfusion (I/R) injury is associated with cardiac dysfunction and depression in sarcolemmal Na+-K+-ATPase activity. This study was undertaken to evaluate the role of proteases in these alterations by subjecting rat hearts to different times of global ischemia, and reperfusion after 45 min of ischemia. Decreases in Na+-K+-ATPase activity at 60 min of global ischemia were associated with augmented activities of both calpain and MMPs and depressed protein content of β1- and β2-subunits, without changes in α1- and α2-subunits of the enzyme. However, reperfusion of ischemic heart produced depression in Na+-K+-ATPase activity, no change in the augmented calpain activity, but decreases in augmented MMP-2 activity and Na+-K+-ATPase content. MDL28170, a calpain inhibitor, was more effective in attenuating I/R-induced alterations than doxycycline, an MMP inhibitor. Incubation of control SL preparation with calpain, unlike MMP-2, depressed Na+-K+-ATPase activity and decreased α1, α2 and β2 without changes in β1. These results support the view that activation of calpain is involved in depressing Na+-K+-ATPase activity and degradation of its subunits in hearts subjected to I/R injury.  en_US
dc.rights info:eu-repo/semantics/openAccess
dc.subject cardiac en_US
dc.subject protease en_US
dc.subject ischemia-reperfusion en_US
dc.subject Na+-K+-ATPase en_US
dc.subject sarcolemma en_US
dc.subject calpain en_US
dc.subject matrix metalloproteinases en_US
dc.title Role of protease activation in sarcolemma Na+-K+-ATPase activity in the heart due to ischemia-reperfusion en_US
dc.type info:eu-repo/semantics/masterThesis
dc.degree.discipline Physiology en_US
dc.contributor.examiningcommittee Freed, Darren (Physiology) Dixon, Ian (Physiology) Czubryt, Michael (Physiology) Moghadasian, Mohammed (Human Nutritional Sciences) en_US
dc.degree.level Master of Science (M.Sc.) en_US
dc.description.note October 2012 en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

View Statistics