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Characterization of anti-ricin monoclonal antibodies and the construction of a chimeric murine-human IgG2/K anti-ricin monoclonal antibody

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dc.contributor.supervisor Berry, Jody (Medical microbiology) en
dc.contributor.author Vendramelli, Robert Matthew
dc.date.accessioned 2011-04-12T17:57:07Z
dc.date.available 2011-04-12T17:57:07Z
dc.date.issued 2011-04-12T17:57:07Z
dc.identifier.uri http://hdl.handle.net/1993/4526
dc.description.abstract Ricin toxin is a very deadly plant protein that is synthesized by the plant Ricinus communis. The molecular structure of ricin toxin places it in a group of similar proteins classified as a Type II RIP due to its heterodimeric construction; it is composed of a toxic A-chain possessing enzymatic action, and a receptor binding B-chain. Monoclonal antibodies were obtained with binding activities against either the A-chain or B-chain, and a surrogate non-toxic ricin analogue, TST10114, was determined to be suitable for characterization of the anti-ricin monoclonal antibodies. One potent anti-ricin A-chain neutralizing monoclonal antibody was chosen for chimerization, RAC18, which exhibited strong binding affinity and neutralizing properties. The constant regions of a human immunoglobulin G2 (IgG2) were used as the backbone for the recombinant chimeric antibody. The resulting chimeric RAC18-huG2 was transiently expressed in human-derived HEK 293F cells, purified, and assessed for binding characteristics and functional attributes. en
dc.format.extent 1969298 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.subject Immunology en
dc.subject ricin en
dc.subject toxin en
dc.subject monoclonal en
dc.subject antibody en
dc.subject chimeric en
dc.title Characterization of anti-ricin monoclonal antibodies and the construction of a chimeric murine-human IgG2/K anti-ricin monoclonal antibody en
dc.degree.discipline Medical Microbiology en
dc.contributor.examiningcommittee Corbett, Cindi (Medical Microbiology) Yao, Xiaojian (Medical Microbiology) Soussi-Gounni, Abdelilah (Immunology) en
dc.degree.level Master of Science (M.Sc.) en
dc.description.note May 2011 en


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