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dc.contributor.supervisor Cardona, Silvia (Microbiology) en
dc.contributor.author Yudistira, Harry
dc.date.accessioned 2010-10-13T19:04:40Z
dc.date.available 2010-10-13T19:04:40Z
dc.date.issued 2010-10-13T19:04:40Z
dc.identifier.uri http://hdl.handle.net/1993/4276
dc.description.abstract Synthetic cystic fibrosis sputum medium (SCFM) is rich in amino acids and supports robust growth of Burkholderia cenocepacia, a member of the Burkholderia cepacia complex (Bcc). Previous work demonstrated that B. cenocepacia phenylacetic acid (PA) catabolic genes are up-regulated during growth in SCFM and are required for full virulence in a Caenorhabditis elegans host model. In this work, we investigated the role of phenylalanine, one of the aromatic amino acids present in SCFM, as an inducer of the PA catabolic pathway. Phenylalanine degradation intermediates were used as sole carbon sources for growth and gene reporter experiments. In addition to phenylalanine and PA, phenylethylamine, and phenylpyruvate could be used as sole carbon sources by wild type B. cenocepacia K56-2 but not by a PA catabolism defective mutant. These intermediates also induced a PA-inducible reporter system. Furthermore, proteomic analysis utilizing iTRAQ were used to study the protein expression of B. cenocepacia K56-2 grown in the amino acid-rich SCFM. Our results showed the over-expression of several proteins involved in amino acid and carbohydrate transport and metabolism. Interestingly, our results also showed the over-expression of flagellin and membrane efflux protein which are involved in the virulence of B. cenocepacia. en
dc.format.extent 1221665 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.rights info:eu-repo/semantics/openAccess
dc.subject Phenylalanine Catabolism en
dc.subject Burkholderia cenocepacia en
dc.title PHENYLALANINE CATABOLISM IN BURKHOLDERIA CENOCEPACIA K56-2 en
dc.type info:eu-repo/semantics/masterThesis
dc.degree.discipline Microbiology en
dc.contributor.examiningcommittee Oresnik, Ivan(Microbiology) Perreault, Hélène (Chemistry) en
dc.degree.level Master of Science (M.Sc.) en
dc.description.note February 2011 en


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