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dc.contributor.supervisor Kobinger, Gary (Medical Microbiology) en
dc.contributor.author Sahib, Mickey M.
dc.date.accessioned 2010-09-10T17:44:18Z
dc.date.available 2010-09-10T17:44:18Z
dc.date.issued 2010-09-10T17:44:18Z
dc.identifier.uri http://hdl.handle.net/1993/4135
dc.description.abstract This thesis describes the production of adenovirus-based vaccines containing codon-optimized genes from Nipah virus and Crimean-Congo Hemorrhagic Fever virus. Genes encoding envelope proteins from Crimean-Congo Hemorrhagic Fever Virus and Nipah Virus were codon-optimized for translation in human cells and constructed using a modified method of non-gapped gene synthesis, while the entire M segment encoding the glycoprotein precursor for Crimean-Congo Hemorrhagic Fever Virus was commercially synthesized. Genes were cloned into recombinant human adenovirus serotype 5 and the resulting viral particles were amplified, titred and analyzed for in vivo efficacy. Results show that a modified method of non-gapped gene synthesis is an effective and efficient method of producing antigen-encoded DNA and at a fraction of the cost and time required for commercial synthesis. Furthermore, adenovirus-based vaccines induce both cellular and humoral immune responses providing for a highly efficacious vaccine during potential disease outbreaks, where time to completion is of utmost importance. This study has shown that recombinant adenoviral vaccines for Crimean-Congo Hemorrhagic Fever virus and Nipah virus can be produced rapidly and efficiently from virtual DNA sequence to optimized recombinant vaccines in just eight months. en
dc.format.extent 1686701 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.subject Crimean en
dc.subject Rift Valley en
dc.subject Nipah en
dc.subject vaccine en
dc.subject immunity en
dc.subject adenovirus en
dc.title Rapid development of optimized recombinant adenoviral vaccines for biosafety level 4 viruses en
dc.degree.discipline Medical Microbiology en
dc.contributor.examiningcommittee Feldmann, Heinz (Medical Microbiology) Babiuk, Shawn (Immunology) en
dc.degree.level Master of Science (M.Sc.) en
dc.description.note October 2010 en


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