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    Structural characterization of phenolic compounds in canola meal: impact of high pressure and temperature

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    Thesis (5.002Mb)
    Date
    2021-07
    Author
    Nandasiri, Hewa Madihe Annakkage Ruchira
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    Abstract
    Canola is the major oilseed crop of Canada. After extraction of the oil, a substantial amount of meal is generated. To date, however, the meal has received little attention from both industry and researchers. Despite its nutrition and functional potential, utilization of canola meal has been limited by its high fiber content and antinutritional components. Nevertheless, it has a rich phenolic profile, that could be a valuable source of nutraceuticals. The presence of large amounts of sinapine and sinapic acid in canola meal and its conversion to canolol has received a lot of attention because of its powerful antioxidant and anticancer properties. What is desired, however, is an effective method for their extraction. The use of pressurized (1,500 psi) high-temperature processing is ideal for the extraction of phenolic compounds from such by-products including the meal. This study investigated the application of accelerated solvent extraction (ASE) as a method for extracting phenolic compounds from canola meal. Different extraction temperatures, solvents, and solvent concentrations were used to establish the optimum extraction conditions for major sinapates and other phenolic compounds. Changes in total phenolics, total flavonoids, and antioxidant capacity were quantified as a measure of extraction efficiency. The ASE method is an efficient method for extracting phenolic compounds from the meal. In addition to ASE, RapidOxy®100, an automated commercial instrument, was adapted as a solvent-free pre-treatment of canola meal prior to the ultrasonic extraction of phenolic compounds. It also proved to be a novel and versatile method for enhancing the extraction of phenolic compounds. Both techniques have the potential for the mainstream production of phenolic-rich extracts as a valuable source of natural bioactive compounds and nutraceuticals from underutilized agricultural by‐products such as canola meal.
    URI
    http://hdl.handle.net/1993/36003
    Collections
    • FGS - Electronic Theses and Practica [25522]
    • Manitoba Heritage Theses [6062]

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