TILRR Promotes Migration of Immune Cells Through Induction of Soluble Inflammatory Mediators

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Date
2020-07-03Author
Kashem, Mohammad
Ren, Xiaoou
Li, Hongzhao
Liang, Binhua
Li, Lin
Lin, Francis
Plummer, Francis
Luo, Ma
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Show full item recordAbstract
TILRR has been identified as an important modulator of inflammatory responses. It is
associated with NF-kB activation and inflammation. Our previous study showed that
TILRR significantly increased the expression of many innate immune responsive genes
and increased the production of several pro-inflammatory cytokines/chemokines by
cervical epithelial cells. In this study, we evaluated the effect of TILRR-induced proinflammatory
cytokines/chemokines on the migration of immune cells. The effect of
culture supernatants of TILRR-overexpressed cervical epithelial cells on the migration
of THP-1 monocytes and MOLT-4 T-lymphocytes was evaluated using Transwell
assay and a novel microfluidic device. We showed that the culture supernatants of
TILRR-overexpressed HeLa cells attracted significantly more THP-1 cells (11–40%,
p = 0.0004–0.0373) and MOLT-4 cells (14–17%, p = 0.0010–0.0225) than that of
controls. The microfluidic device-recorded image analysis showed a significantly
higher amount with a longer mean cell migration distance of THP-1 (p < 0.0001–0.0180)
and MOLT-4 (p < 0.0001–0.0025) cells was observed toward the supernatants of
TILRR-overexpressed cervical epithelial cells compared to that of the controls. Thus,
the cytokines/chemokines secreted by the TILRR-overexpressed cervical epithelial cells
attracted immune cells, such as monocytes and T cells, and may potentially influence
immune cell infiltration in tissues.