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    TILRR Promotes Migration of Immune Cells Through Induction of Soluble Inflammatory Mediators

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    Date
    2020-07-03
    Author
    Kashem, Mohammad
    Ren, Xiaoou
    Li, Hongzhao
    Liang, Binhua
    Li, Lin
    Lin, Francis
    Plummer, Francis
    Luo, Ma
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    Abstract
    TILRR has been identified as an important modulator of inflammatory responses. It is associated with NF-kB activation and inflammation. Our previous study showed that TILRR significantly increased the expression of many innate immune responsive genes and increased the production of several pro-inflammatory cytokines/chemokines by cervical epithelial cells. In this study, we evaluated the effect of TILRR-induced proinflammatory cytokines/chemokines on the migration of immune cells. The effect of culture supernatants of TILRR-overexpressed cervical epithelial cells on the migration of THP-1 monocytes and MOLT-4 T-lymphocytes was evaluated using Transwell assay and a novel microfluidic device. We showed that the culture supernatants of TILRR-overexpressed HeLa cells attracted significantly more THP-1 cells (11–40%, p = 0.0004–0.0373) and MOLT-4 cells (14–17%, p = 0.0010–0.0225) than that of controls. The microfluidic device-recorded image analysis showed a significantly higher amount with a longer mean cell migration distance of THP-1 (p < 0.0001–0.0180) and MOLT-4 (p < 0.0001–0.0025) cells was observed toward the supernatants of TILRR-overexpressed cervical epithelial cells compared to that of the controls. Thus, the cytokines/chemokines secreted by the TILRR-overexpressed cervical epithelial cells attracted immune cells, such as monocytes and T cells, and may potentially influence immune cell infiltration in tissues.
    URI
    http://hdl.handle.net/1993/35795
    DOI
    10.3389/fcell.2020.00563
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    • Rady Faculty of Health Sciences Scholarly Works [1296]
    • University of Manitoba Scholarship [1981]

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