• Libraries
    • Log in to:
    View Item 
    •   MSpace Home
    • University of Manitoba Researchers
    • University of Manitoba Scholarship (login required)
    • View Item
    •   MSpace Home
    • University of Manitoba Researchers
    • University of Manitoba Scholarship (login required)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    A simplified silver phosphate extraction method for oxygen isotope analysis of bioapatite

    Thumbnail
    View/Open
    Shabaga et al 2018 accepted.pdf (456.7Kb)
    Date
    2018-06-21
    Author
    Shabaga, BM
    Gough, H
    Fayek, M
    Hoppa, RD
    Metadata
    Show full item record
    Abstract
    Rationale: Although phosphatic materials are chemically complex and are prone to exchange oxygen isotopes with their environments, the phosphate (PO43-) component of these materials is robust and retains its original oxygen isotopic composition. As a result, there are currently several methods for the isolation of phosphate oxygen through the precipitation of silver phosphate (Ag3PO4). However, some of these techniques produce Ag3PO4 of questionable purity, while nearly all are lengthy and/or require relatively large sample sizes. Methods: Five milligrams of bioapatite from modern cow teeth (enamel and cementum) were pre-treated for removal of organic material prior to digestion in 2M HF. The digested samples were titrated with silver ammine solution at 50°C to precipitate Ag3PO4. Oxygen isotopic data were collected using a Thermal Combustion Elemental Analyzer (TC/EA) paired with a Delta VPlus isotope ratio mass spectrometer via a ConFlo III universal interface. Results: The quality of Ag3PO4 is dependent on effective removal of organic material and the volume of silver ammine solution used during titration. A two-step pre-treatment of 2.5% NaOCl, followed by a 0.125M NaOH solution is the most effective treatment for the removal of organic material from both enamel and cementum. Optimal yields of Ag3PO4 were achieved using 1.8 mL of silver ammine solution. The reproducibility of the phosphate δ18O compositions ranges from 0.3 to 0.4‰ (1σ) for modern cow teeth. Conclusions: We present a simplified method for phosphate extraction from organic-rich phosphatic material. Our method gave reproducible 18O values for enamel and cementum from cows’ teeth.
    URI
    http://hdl.handle.net/1993/35157
    DOI
    10.1002/rcm.8149
    Collections
    • University of Manitoba Scholarship (login required) [171]

    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    Theme by 
    Atmire NV
     

     

    Browse

    All of MSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    Login

    Statistics

    View Usage Statistics

    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    Theme by 
    Atmire NV