Cardiac mitochondrial respiration and ROS production and consumption with changing temperature in: Oncorhynchus mykiss, Cyprinus carpio, and Acipenser fulvescens
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Date
2018
Authors
Wiens, Lilian Maria
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Abstract
Cardiac mitochondrial metabolism of Oncorhyncus mykiss; Cyprinus carpio; and Acipenser fulvescens, was examined with increasing temperature. Experiments assayed mitochondrial oxygen consumption, and ROS, reactive oxygen species production and consumption. Rates of two mitochondrial enzymes were determined (citrate synthase and cytochrome c oxidase). Oxygen consumption rises with temperature for C. carpio and A. fulvescens, but not for O. mykiss. Respiratory control ratio decreases with increasing temperature for all but C. carpio. ROS production increases with temperature for A. fulvescens, with O. mykiss and C. carpio being more variable in their response. ROS consumption does not change with temperature in C. carpio, and O. mykiss mitochondria, with A. fulvescens increasing. Interspecific differences across species disappear when rates are expressed as cytochrome c oxidase activity. The ratio between ROS production and consumption, and fractional electron leak increased with temperature, which may be an indication of incumbent stress due to acute temperature warming.
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Keywords
Mitochondria, ROS production, ROS consumption, Fish metabolism, Fish mitochondria bioenergetics, Comparative physiology, Fish heart, Lake sturgeon, Rainbow trout, Common carp