The function of the transcription factor HLH-6 in the esophageal gland cells of root knot nematodes (meloidogyne)
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Date
2016
Authors
Loewen, Royden A.
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Abstract
Root-knot nematodes (RKN) are plant parasites, and a major agricultural pest. RKN are adept at invading and feeding on plant tissue with secretions they release from their gland cells. This thesis compares glandular development in Meloidogyne to the model nematode, Caenorhabditis elegans. Pharyngeal and gland important transcription factors, PHA-4 and HLH-6, respectively, were examined for their role in regulation of parasite-specific genes. Homologues of these genes were identified and sequenced from M. incognita. Phylogenetic analyses revealed a new PPN-specific gene, hlh-6-like. Bioinformatic comparisons of the homologues revealed conservation of the DNA-binding motifs of Ce-HLH-6 and Mi-HLH-6 proteins, as well as conservation of their promoter regions. While HLH-6 antibodies proved inconclusive, expression assays revealed expectant levels of PHA-4 and HLH-6 in Meloidogyne as compared to C. elegans. In identifying genes essential for parasitism we are providing new targets for knockdown during RKN infection.
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Keywords
Nematode, Parasite, Development, Genetics, Root-knot