Characterize the anti-HIV-1 activity of a kinase inhibitor kenpaullone and the HIV-1 integrase association with DIC1 and DYNLT1

Loading...
Thumbnail Image
Date
2015
Authors
Chen, Bihe
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Advances in the antiretroviral therapy (ART) have dramatically reduced the death rate from human immunodeficiency virus type 1 (HIV-1) induced acquired immune deficiency syndrome (AIDS). However, it is still necessary to develop anti-HIV-1 new drugs. In this study, two projects were conducted and may contribute to the new drug development. The first project is focused on characterizing the anti-HIV activity of a kinase inhibitor Kenpaullone (Ken). We found a cyclin dependent kinase (CDK) and glycogen synthase kinase-3β (GSK-3β) inhibitor named Ken can significantly inhibit HIV-1 replication. Mechanistic analysis by RT-PCR revealed that Ken inhibited HIV-1 replication by disrupting transcription possibly through CDK-dependent pathways. The second project is focused on understanding the association between HIV-1 integrase (IN) and dynein components. Our investigation indicated that HIV-1 IN is associated with DIC1 and DYNLT1. Further investigation this IN/dynein component association may help to reveal new anti-HIV targets.
Description
Keywords
HIV-1, Kinase, Kenpaullone, Integrase, Dynein
Citation