Endogenous Phenolics from Expeller-pressed Canola Oil Refining Byproducts: Evaluation of Antioxidant Activities in Cell Culture and Deep-fat Frying Models

Loading...
Thumbnail Image
Date
2014, 2014, 2014
Authors
Chen, Yougui
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of agricultural and food chemistry
European Journal of Lipid Science and Technology
Journal of the American Oil Chemists' Society
Abstract
Sinapic acid derivatives and tocopherols in refining byproducts of commercially produced expeller-pressed canola oils were characterized and isolated. Additionally, the antioxidant activities of the phenolics were examined by three systems including an in vitro non-biological related assay, a cellular assay and a deep-fat frying model. Sinapic acid (SA: 42.9 µg/g), Sinapine (SP: 199 µg/g), and Canolol (CAN: 344 µg/g) were found in different byproducts of canola oil refining, namely, soapstock, spent bleaching clay, and wash-water, respectively. Tocopherols (3.75 mg/g) and other non-identified phenolic compounds (2.7 mg /g) were found in deodistillates (DDL). CAN and DDL revealed significant protection effect (p<0.05) against hydrogen peroxide induced oxidation in two mammalian cell lines. The results of deep-fat frying studies indicated positive effects of CAN and DDL in preventing lipid oxidation. The canola oils fortified with DDL and CAN showed a considerable reduction (p < 0.05) in oxidation products of lipid after frying.
Description
Keywords
canola oil, refining, phenolics, antioxidant activities, cell culture, deep-fat frying
Citation
Chen, Y., Thiyam-Hollander, U., Barthet, V. J., & Aachary, A. A. (2014). Value Added Potential of Expeller-Pressed Canola Oil Refining: Characterization of Sinapic Acid Derivatives and Tocopherols from By-Products. Journal of agricultural and food chemistry. 62(40), 9800-9807.
Aachary, A. A., Chen, Y., Eskin, N. A.M., & Thiyam‐Hollander, U. (2014). Effect of crude canolol and canola distillate extracts on the stability of refined canola oil during deep-fat frying. European Journal of Lipid Science and Technology. 116 (11), 1467-1476.
Matthäus, B., Pudel, F., Chen, Y., Achary, A., & Thiyam-Holländer, U. (2014). Impact of Canolol-Enriched Extract from Heat-Treated Canola Meal to Enhance Oil Quality Parameters in Deep-Frying: a Comparison with Rosemary Extract and TBHQ-Fortified Oil Systems. Journal of the American Oil Chemists' Society, 91(12), 2065-2076.