A standard sample size of 60 fish was the objective for each of the target species of fish. A 60 fish sample size provides a 95% confidence level that a pathogen in an infected fish will be detected given in a 5% presumed prevalence of infection in that population ( >2000). Fish were captured by used of experimental gill nets, and modified Fyke nets. After capture the fish were transported to a temporary field laboratory at Devil's Lake and held alive with Devil's Lake water until time of necropsy, examination, and sample collection.