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dc.contributor.supervisorMcVetty, Peter B.E. (Plant Science)en
dc.contributor.authorRahman, Md. Mukhlesur
dc.date.accessioned2007-09-28T14:36:33Z
dc.date.available2007-09-28T14:36:33Z
dc.date.issued2007-09-28T14:36:33Z
dc.identifier.urihttp://hdl.handle.net/1993/2846
dc.description.abstractMolecular markers for seed quality traits including erucic acid content genes, seed coat color genes in Brassica napus and seed coat color genes in B. rapa were developed. A single base change in the Bn-FAE1.1 gene in the A genome and a two-base deletion in the Bn-FAE1.2 gene in the C genome produce the nearly zero content of erucic acid observed in canola. The single base change was detected as single nucleotide polymorphic (SNP) marker with an ABI SNaPshot kit. A multiplexing primer set was designed by adding a polyT to the 5´ primer end to increase SNP detection throughput through sample pooling. The two-base deletion in the C genome gene was detected as a sequence characterized amplified region (SCAR) marker in an ABI 3100 Genetic analyzer. To increase the throughput, one genome specific primer was labeled with four fluorescence dyes and combined with 20 different primers to produce PCR products with different fragment sizes. These multiplexed high throughput molecular markers have been successfully implemented in our canola/rapeseed breeding programs. Trigenic inheritance was observed for seed coat color in B. napus. Three Sequenced Related Amplified Polymorphism (SRAP) markers very closely linked to the three different seed coat color genes were developed. Chromosome-walking technology was used to convert the SRAP marker into a SCAR marker and a SNP marker. Subsequently, the first seed coat color gene (Bn1) marker was converted into a SCAR marker, and the second seed coat color gene (Bn2) marker was converted into a SNP marker. Digenic inheritance was observed for seed coat color genes in B. rapa. A SRAP marker was identified as being tightly linked to the major seed coat color gene (Br1). The SRAP marker was sequenced and extended sequences were obtained using chromosome-walking technology. The flanking sequences of the SRAP marker contained 24 SNPs and a 12-bp deletion position that allowed the marker to be converted into a co-dominant SNP marker and a co-dominant SCAR marker, respectively. The SCAR marker was detected in the ABI 3100 genetic analyzer with four fluorescently labeled M13 primers integrated with different SCAR primers, which permitted pooling of PCR samples for high throughput detection.en
dc.format.extent2221261 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMolecular markeren
dc.subjecterucic acid genesen
dc.subjectseed coat color genesen
dc.subjectBrassica napusen
dc.subjectBrassica rapaen
dc.subjectSRAP, SNP, SCARen
dc.titleDevelopment of molecular markers for marker assisted selection for seed quality traits in oilseed rapeen
dc.typeinfo:eu-repo/semantics/doctoralThesis
dc.typedoctoral thesisen_US
dc.degree.disciplinePlant Scienceen_US
dc.contributor.examiningcommitteeLi, Genyi (Plant Science) Schroeder, Dana (Biological Sciences) Pauls, Peter K. (University of Guelph)en
dc.degree.levelDoctor of Philosophy (Ph.D.)en_US
dc.description.noteOctober 2007en


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