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dc.contributor.author Gungormusler-Yilmaz, Mine
dc.contributor.author Shamshurin, Dmitry
dc.contributor.author Grigoryan, Marine
dc.contributor.author Taillefer, Marcel
dc.contributor.author Spicer, Victor
dc.contributor.author Krokhin, Oleg V
dc.contributor.author Sparling, Richard
dc.contributor.author Levin, David B
dc.date.accessioned 2014-09-01T15:25:36Z
dc.date.available 2014-09-01T15:25:36Z
dc.date.issued 2014-08-30
dc.identifier.citation AMB Express. 2014 Aug 30;4(1):63
dc.identifier.uri http://hdl.handle.net/1993/23921
dc.description.abstract Abstract Higher initial glycerol loadings (620 mM) have a negative effect on growth and 1,3-propanediol (1,3-PDO) synthesis in Clostridium butyricum DSM 10702 relative to lower initial glycerol concentrations (170 mM). To help understand metabolic shifts associated with elevated glycerol, protein expression levels were quantified by LC/MS/MS analyses. Thirty one (31) proteins involved in conversion of glycerol to 1,3-PDO and other by-products were analyzed by multiple reaction monitoring (MRM). The analyses revealed that high glycerol concentrations reduced cell growth. The expression levels of most proteins in glycerol catabolism pathways were down-regulated, consistent with the slower growth rates observed. However, at high initial glycerol concentrations, some of the proteins involved in the butyrate synthesis pathways such as a putative ethanol dehydrogenase (CBY_3753) and a 3-hydroxybutyryl-CoA dehydrogenase (CBY_3045) were up-regulated in both exponential and stationary growth phases. Expression levels of proteins (CBY_0500, CBY_0501 and CBY_0502) involved in the reductive pathway of glycerol to 1,3-PDO were consistent with glycerol consumption and product concentrations observed during fermentation at both glycerol concentrations, and the molar yields of 1,3-PDO were similar in both cultures. This is the first report that correlates expression levels of glycerol catabolism enzymes with synthesis of 1,3-PDO in C. butyricum. The results revealed that significant differences in the expression of a small subset of proteins were observed between exponential and stationary growth phases at both low and high glycerol concentrations.
dc.title Reduced catabolic protein expression in
dc.type Journal Article
dc.language.rfc3066 en
dc.description.version Peer Reviewed
dc.rights.holder Mine Gungormusler-Yilmaz et al.; licensee BioMed Central Ltd.
dc.date.updated 2014-09-01T15:25:39Z
dc.identifier.doi http://dx.doi.org/10.1186/s13568-014-0063-6


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