Show simple item record

dc.contributor.authorPatrick, Megan Kathleenen_US
dc.date.accessioned2007-05-22T15:14:41Z
dc.date.available2007-05-22T15:14:41Z
dc.date.issued2001-05-01T00:00:00Zen_US
dc.identifier.urihttp://hdl.handle.net/1993/2055
dc.description.abstractCurrently, little is known about the genetic and biological functions of avian reovirus (ARV), an atypical member of the family 'Reoviridae ' and the prototype of all non-enveloped syncytia-inducing viruses. In this study, we created temperature sensitive ('ts') mutants by chemical mutagenesis of ARV strain 138. We developed a novel efficiency of lysis (EOL) technique to screen for temperature sensitivity and used the classical efficiency of plating (EOP) assay to identify 17 ARV 'ts ' mutants. Pairwise mixed infection of these mutants and evaluation of recombinant progeny 'ts' status led to their organization into 7 recombination groups. This indicates that these new groups of ARV mutants represent the majority of the ARV genome. To phenotypically characterize each mutant and localize the effects of the 'ts' lesion within the viral lifecycle, the ability to synthesize double stranded RNA (dsRNA) at permissive (33.5C) and non-permissive temperature (39.5C) was analyzed. (Abstract shortened by UMI.)en_US
dc.format.extent6296056 bytes
dc.format.extent184 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleThe generation and characterization of avian reovirus temperature sensitive mutantsen_US
dc.typeinfo:eu-repo/semantics/masterThesis
dc.typemaster thesisen_US
dc.degree.disciplineMedical Microbiologyen_US
dc.degree.levelMaster of Science (M.Sc.)en_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record