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dc.contributor.authorTurenne, Christine Yvetteen_US
dc.date.accessioned2007-05-17T12:33:39Z
dc.date.available2007-05-17T12:33:39Z
dc.date.issued1998-09-01T00:00:00Zen_US
dc.identifier.urihttp://hdl.handle.net/1993/1356
dc.description.abstractBacteremia and fungemia contribute significantly to the mortality and morbidity of patients. The use of blood cultures is not rapid, sensitive or specific enough to identify or exclude all causes of a febrile episode, resulting in empiric administration of broad-spectrum antibiotics based on clinical grounds. To overcome these limitations, we have made use of the sequence variability of the 16S ribosomal DNA (rDNA) of bacteria and the intergenic transcribed spacer region (ITS2) of fungi for rapid DNA amplification, detection and identification of pathogens from positive blood cultures using an automated fluorescent capillary electrophoresis sequencer. Amplification was tested for cross-reactivity with human DNA, effect of prolonged sample storage and presence of various antibiotics. The SSCP patterns of the most commonly isolated bacteria from blood cultures and the ITS2 length of 47 various fungal species were determined using control strains. The molecular identification of 304 blood culture positive clinical specimens and 101 seeded yeast blood cultures, using the determined SSCP patterns or ITS2 length as reference, was compared to conventional identification in a double-blinded fashion. (Abstract shortened by UMI.)en_US
dc.format.extent6573164 bytes
dc.format.extent184 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleThe use of the 16S rRNA gene and the internal transcribed spacer, ITS2, region for rapid and specific identification of bacteria and fungi from clinical specimensen_US
dc.typeinfo:eu-repo/semantics/masterThesis
dc.typemaster thesisen_US
dc.degree.disciplineMedical Microbiologyen_US
dc.degree.levelMaster of Science (M.Sc.)en_US


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