Hepatocyte [H-3]-palmitate uptake: effect of albumin surface charge modification

Simple item page
dc.contributor.authorBurczynski, FJ
dc.contributor.authorWang, GQ
dc.contributor.authorElmadhoun, B
dc.contributor.authorShe, YM
dc.contributor.authorRoberts, MS
dc.contributor.authorStanding, KG
dc.date.accessioned2007-10-30T13:41:37Z
dc.date.available2007-10-30T13:41:37Z
dc.date.issued2001-10-31
dc.description.abstractThe role of plasma proteins on the cellular uptake of lipophilic substrates has perplexed investigators for many years. We tested the hypothesis that an ionic interaction between the protein-ligand complex and hepatocyte surface may be responsible for supplying more ligand to the cell for uptake. The surface-charged groups on albumin were modified to yield proteins having a range of isoelectric points (ALB, ALBs, ALBm, ALBe had values of 4.8-5.0, 4.5-4.7, 3.0-3.5, 8.4-8.6, respectively). [H-3]-Palmitate uptake studies were performed with adult rat hepatocyte suspensions using similar unbound ligand fractions in the presence of the different binding proteins. Mass spectrometry, isoelectric focusing (pI), and heptane : water partitioning were used to determine protein molecular weight, pI, and protein-palmitate equilibrium binding constant, respectively. Hepatocyte [H-3]-palmitate clearance in the presence of ALBs and ALBm were significantly lower (p < 0.05) than ALB, whereas [H-3]-palmitate clearance in the presence of ALBe was significantly higher (p < 0.05) than ALB. The data were consistent with the notion that ionic interactions between extracellular protein-ligand complexes and the hepatocyte surface facilitate the uptake of long-chain fatty acids.en
dc.format.extent411902 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.citation0008-4212; CAN J PHYSIOL PHARMACOL, OCT 2001, vol. 79, no. 10, p.868 to 875.en
dc.identifier.doihttp://dx.doi.org/10.1139/y01-072
dc.identifier.urihttp://hdl.handle.net/1993/2945
dc.language.isoengen_US
dc.rightsNo part of the NRC Research Press electronic journals may be reproduced, stored, or transmitted in any form or by any means, without the written permission of the publisher, except as stated below. Under the Canadian Copyright Act, individuals may download or print single copies of articles for personal research or study. Any person may reproduce short excerpts from articles in the journals for any purpose that respects the moral rights of authors, provided that the source is fully acknowledged. As a courtesy, the consent of authors of such material should be obtained directly from the author. Authorization to reproduce items for other than personal research or study, as stated above, may be obtained via Access © upon payment of the copyright fee of $10.00 per copy. NRC Research Press also extends certain additional rights to authors. The above rights do not extend to copying or reproduction for general distribution, for advertising or promotional purposes, for creating new collective works, or for resale. For such copying or reproduction, arrangements must be made with NRC Research Press.en
dc.rightsopen accessen_US
dc.statusPeer revieweden
dc.subjectelectromagnetic shieldingen
dc.subjectinduced solid conductorsen
dc.subjectquasi-stationary fieldsen
dc.titleHepatocyte [H-3]-palmitate uptake: effect of albumin surface charge modificationen
dc.typejournal articleen_US
Files
Collections
University of Manitoba Scholarship (login required)