Pseudomonas aeruginosa type III secretion system: regulation and potential role in interspecies interaction

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dc.contributor.authorZhao, Yichen
dc.contributor.examiningcommitteeScott, James Elliott (Oral Biology) Chelikani, Prashen (Oral Biology) Cardona, Silvia (Microbiology)en_US
dc.contributor.supervisorDuan, Kangmin (Oral Biology)en_US
dc.date.accessioned2014-08-26T20:16:37Z
dc.date.available2014-08-26T20:16:37Z
dc.date.issued2014-08-26
dc.degree.disciplineOral Biologyen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractPseudomonas aeruginosa causes various infections in humans, animals and plants. Type III secretion system (T3SS) is one of the essential virulence factors used by P. aeruginosa. In this study, a previously uncharacterized gene PA0466 and its role in T3SS regulation have been examined. The results indicate that PA0466 is a novel T3SS regulator. It regulates T3SS directly through an unknown pathway and has a minor effect on the GacA-RsmA pathway. Besides the role in the interaction between the pathogen and the host, T3SS may also play a role in the interspecies interaction. A real-time PCR based Competitive Index (CI) assay was used to compare the wild type and T3SS mutant with and without the presence of Staphylococcus spp.. The results indicate that PAO1 was more competitive than exsA mutant and the difference was even bigger in the presence of Staphylococcus, suggesting T3SS may play a significant role in bacterium-bacterium interaction.en_US
dc.description.noteOctober 2014en_US
dc.identifier.urihttp://hdl.handle.net/1993/23889
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectPseudomonas aeruginosaen_US
dc.subjecttype III secretion systemen_US
dc.subjectPA0466 over-expressionen_US
dc.subjectinterspecies interactionen_US
dc.titlePseudomonas aeruginosa type III secretion system: regulation and potential role in interspecies interactionen_US
dc.typemaster thesisen_US
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