Nuclear DNA amount determination and cytology of some afroalpine angiosperms in Ethiopia

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dc.contributor.authorKibret, Kidist
dc.date.accessioned2023-03-10T20:43:06Z
dc.date.available2023-03-10T20:43:06Z
dc.date.issued2007-04
dc.date.submitted2023-03-08T17:17:04Zen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractThis study includes five species of the afroalpine flora- Cardamine obliqua (Brassicaceae), Cotula cryptocephala (Asteraceae), Deschampsia caespitosa (Poaceae), Ranunculus oreophytus and Ranunculus stagnalis (Ranunculaceae) collected from Bale and Simen Mountains. Estimates of 2C-values and DNA-ploidy analysis were done for C. cryptocephala, D. caespitosa, R. oreophytus and R. stagnalis from leaf tissue using flow cytometry. Chromosome counting from mitotic root tips was done for C. cryptocephala, D. caespitosa, R. oreophytus, R. stagnalis and C. obliqua. Mean 2C-values were found to be 10.2 pg, 9.2 pg, 27.5 pg and 14.2 pg for C. cryptocephala, D. caespitosa, R. oreophytus and R. stagnalis, respectively. Chromosome numbers found were the same as earlier reports, 2n=62, 26, 32, 28 for C. obliqua, D. caespitosa, R. oreophytus and R. stagnalis, respectively but for C. cryptocephala 2n= 64 is a new report. For all species analyzed by flow cytometry, no intraspecific DNA-ploidy level difference was found whereas slight intraspecific 2C-value variation up to 1.047-fold was found, which could be either artefactual or real intraspecific variation correlated with molecular, morphological or environmental factors. These results indicate that further morphological, cytological, flow cytometric and molecular investigations of the afroalpine flora will provide data that answer many important biological and evolutionary questions.en_US
dc.identifier.urihttp://hdl.handle.net/1993/37200
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectNuclear DNA amounten_US
dc.subjectDNA ploidyen_US
dc.subjectchromosome countsen_US
dc.subjectafroalpineen_US
dc.subjectplant evolutionen_US
dc.titleNuclear DNA amount determination and cytology of some afroalpine angiosperms in Ethiopiaen_US
dc.typemaster thesisen_US
local.author.affiliationFaculty of Agricultural and Food Sciences::Department of Plant Scienceen_US
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