Introgression of blackleg resistance genes from related Brassica species to B. napus

Simple item page
dc.contributor.authorShah, Keval Pravinchandra
dc.contributor.examiningcommitteeMcCartney, Curt (Plant Science)
dc.contributor.examiningcommitteeBandara, Nandika (Food and Human Nutritional Sciences)
dc.contributor.examiningcommitteeChen, Guanqun (Gavin) (University of Alberta)
dc.contributor.supervisorDuncan, Robert
dc.contributor.supervisorStasolla, Claudio
dc.date.accessioned2024-05-17T20:09:16Z
dc.date.available2024-05-17T20:09:16Z
dc.date.issued2024-04-08
dc.date.submitted2024-04-19T21:01:32Zen_US
dc.degree.disciplinePlant Science
dc.degree.levelDoctor of Philosophy (Ph.D.)
dc.description.abstractCanola (Brassica napus L.) is one of the most important oilseed crops, contributing more than $26 billion annually to the Canadian economy. Blackleg is caused by fungal plant pathogen Leptosphaeria maculans (Desm) Ces. et de Not. In canola, blackleg disease causes more than $1 billion in yield losses globally. There are different disease management strategies, and using resistant cultivars is one of the economically viable and environmentally sustainable approaches. There are reports of a breakdown of blackleg disease resistance in Australia, France, and Canada due to severe pathogen pressure. Twenty-two blackleg-specific resistance genes have been identified in different Brassica species. Out of these, the B-genome blackleg-specific resistance genes provide resistance throughout a plant's life. This thesis addresses the identification of putative novel sources of resistance from Brassica juncea and hexaploid Brassica into B. napus. In the first project, different B. juncea UM lines were used to identify a high level of resistance against blackleg disease and were screened with L maculans isolate 03-15-03 (AvrLm2, AvrLm3, AvrLm5-9, AvrLm6, AvrLm10, and AvrLm11) and PG4-1-M (AvrLm2, AvrLm5-9, AvrLm6, AvrLm10, and AvrLm11). Plants were inoculated with different L. maculans (AvrLm3 and avrLm3; AvrLm5 AvrLm6, AvrLm5 avrLm6, and avrLm5 avrLm6) isolates to identify the novel source of resistance in Brassica napus Westar x Brassica juncea UM3073 (UMBJ16) genotypes. For genotyping, UMBJ16 genotypes were tested for all the available blackleg-specific resistance gene-linked Kompetitive Allele-Specific PCR (KASP) (Rlm1, Rlm2, Rlm3, Rlm4, Rlm7, Rlm9, LepR1, LepR2, LepR3, and LepR4) and Simple Sequence Repeats (SSR) markers (Rlm6 and rjlm2). In the second project, the B-genome blackleg-specific resistance genes were introgressed from hexaploid Brassica into B. napus. Similar phenotyping and genotyping approaches were followed to identify the putative novel source of resistance against L. maculans isolate 03-15-03 and PG4-1-M in Brassica napus Westar x hexaploid Brassica crosses (BNHB16). This resulted in the identification of a putative novel source of resistance against L. maculans isolate PG4-1-M in UMBJ16 and BNHB16 genotypes. This newly developed germplasm will help to develop blackleg-resistant breeding material in canola and rapeseed breeding programs.
dc.description.noteOctober 2024
dc.description.sponsorshipUniversity of Manitoba Canola Council of Canada Agriculture and Agri-Food Bunge Nutrien
dc.identifier.urihttp://hdl.handle.net/1993/38242
dc.language.isoeng
dc.rightsopen accessen_US
dc.subjectBrassica napus
dc.subjectBrassica juncea
dc.subjecthexaploid Brassica
dc.subjectKASP marker
dc.subjectSSR marker
dc.subjectblackleg
dc.subjectcanola
dc.subjectLeptosphaeria maculans
dc.subjectresistance breeding
dc.titleIntrogression of blackleg resistance genes from related Brassica species to B. napus
dc.typedoctoral thesisen_US
local.subject.manitobano
project.funder.nameNational Science and Engineering Research Council
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Shah_Keval.pdf
Size:
2.19 MB
Format:
Adobe Portable Document Format
Description:
There are two manuscripts in the submission stage which refers to chapter 3 and 4.
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
770 B
Format:
Item-specific license agreed to upon submission
Description:
Download
Collections
FGS - Electronic Theses and Practica