Identifying the functional relevance of CD271 and CD133 in medulloblastoma tumor formation
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Date
2012-11-22
Authors
Aiken, Christopher
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Abstract
Medulloblastoma (MB) is the most common primary malignant pediatric brain
tumor. The relationship between stem cell function and invasiveness has not been
investigated in MB. We have dissected MB heterogeneity and compared the capacity for
self-renewal and invasion. Our laboratory has identified a combination of 3 cell surface
markers that can be used to select for cells with a high self renewal capacity or high
invasive capacity. CD271 and CD24 cell surface markers were shown to select for cells
that exhibited higher self-renewal capacity whereas CD133 selected for higher invasive
capacity. The next step was to confirm these findings in vivo. A mouse model was
developed and optimized in the laboratory using NOD SCID mice. Initial data has shown
mice injected with higher unsorted cell numbers develop tumor symptoms earlier than
mice injected with less cells. Additionally, CD271+/133- cells appear to develop tumors
at a faster rate than CD271-/CD133- cells.
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Medicine