Polymorphisms in the sodium-dependent ascorbate transporter gene SLC23A1 are associated with susceptibility to Crohn’s disease
dc.contributor.author | Amir Shaghaghi, Mandana | |
dc.contributor.author | Serrano León, Alejandra | |
dc.contributor.author | El-Gabalawy, Hani | |
dc.date.accessioned | 2015-05-25T15:14:04Z | |
dc.date.available | 2015-05-25T15:14:04Z | |
dc.date.issued | 2014 | |
dc.description.abstract | Abstract BACKGROUND: Crohn disease (CD) and ulcerative colitis (UC) are 2 common inflammatory bowel diseases (IBDs) associated with intestinal inflammation and tissue damage. Oxidative stress is suggested to play a major role in the initiation and progression of IBD. Vitamin C (ascorbate, ascorbic acid) supplementation has reduced oxidative stress in persons with IBD. The role of ascorbate transporters in IBD remains to be determined. SLC23A1 is a major ascorbate transporter in the intestinal tract, and some of its genetic variants have been associated with severely decreased ascorbate transport and lower systemic concentrations. OBJECTIVE: This study aimed to determine whether common genetic variants in the vitamin C transporter SLC23A1 are associated with the risk of IBD. DESIGN: Genomic DNA samples from patients with CD (n = 162) and UC (n = 149) from the Manitoba IBD Cohort Study and ethnically matched controls (n = 142) were genotyped for 3 SLC23A1 polymorphisms (rs6596473, rs33972313, and rs10063949) by using TaqMan assays. RESULTS: Variation at rs10063949 (G allele for heterozygote and homozygote) was associated with increased susceptibility to CD (OR: 2.54; 95% CI: 1.38, 4.66; OR: 4.72; 95% CI: 2.53, 8.81; P < 0.0001; respectively). A strong linkage disequilibrium (LD) was observed across the SLC23A1 region (variation rs6596473 with rs10063949) for CD and UC (D' = 0.94 and 0.96, respectively). The risk alleles confirmed a haplotype (CGG) that is carried more in CD patients (65.3%, P < 0.0001) than in controls (43.5%). CONCLUSIONS: A genetic variant (rs10063949-G) in the SLC23A1 ascorbate transporter locus was identified and is associated with an increased risk of CD in a white Canadian IBD cohort. The presented evidence that SLC23A1 variations can modulate the risk of CD has implications for understanding ascorbate transport in CD patients and provides a novel opportunity toward individualized nutritional therapy for patients carrying the disease-associated genotype. | en_US |
dc.identifier.citation | Am J Clin Nutr. 2014 Feb;99(2):378-83. | en_US |
dc.identifier.doi | 10.3945/ajcn.113.068015 | |
dc.identifier.uri | http://hdl.handle.net/1993/30527 | |
dc.language.iso | eng | en_US |
dc.publisher | American Journal of Clinical Nutrition | en_US |
dc.relation.ispartofseries | 99(2);378-83 | |
dc.rights | open access | en_US |
dc.subject | Crohns | en_US |
dc.subject | Nutrition | en_US |
dc.subject | Bernstein | en_US |
dc.subject | Polymorphisms | en_US |
dc.subject | Sodium-independent | en_US |
dc.title | Polymorphisms in the sodium-dependent ascorbate transporter gene SLC23A1 are associated with susceptibility to Crohn’s disease | en_US |
dc.type | Article | en_US |
dc.type | Dataset | en_US |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- AJCN-Shaghagi manuscript for SLC23A1 final (1).pdf
- Size:
- 354 KB
- Format:
- Adobe Portable Document Format
- Description:
- Main Article
License bundle
1 - 1 of 1
Loading...
- Name:
- license.txt
- Size:
- 2.27 KB
- Format:
- Item-specific license agreed to upon submission
- Description: