(Introduction) The number of Physician Assistants (PA) in Canadian healthcare will grow as the population increases and ages. The closure of the Canadian Armed Forces Physician Assistant Education Program will increase the direct entry of civilian-educated PAs into military service. Knowing the factors related in military or civilian education and practice is essential in understanding this human resource. (Methods) Information for this qualitative study was gathered through structured interviews to develop and explore the themes occurring during the Canadian Armed Forces Physician Assistant’s journey through their career, education deployment, and transition to civilian employment. (Results) Civilian PAs joining the military need to understand that the military is a controlled environment that requires flexibility and adaptability, but among the benefits is a high level of comradery. Role differences were noted in military and civilian practice and explored the experiences, practice environments, teamwork, resiliency, flexibility, limited range of patient ages, and medical conditions limited clinical experiences. (Conclusion) The transitions into civilian practice by military PAs resulted in a steep learning curve related to the health concerns more common to civilian populations. Still, they were backed by the confidence and attributes those individuals developed in the military. Regarding work environments, the military requires and offers unique experiences and posting to remote locations, naval ships, and overseas, which can result in autonomous practice only found in specific rural civilian areas.