Pre-harvest sprouting (PHS), which is defined as the germination of seeds prior to harvest under moist conditions, occurs in wheat and is closely related to seed dormancy. Low levels of seed dormancy result in pre-harvest sprouting, which adversely impacts wheat grain yield and end-use quality; for example, sprouted seeds have poor quality that is unsuitable for baking. On the other hand, high levels of seed dormancy cause a delay in germination and seedling establishment. Therefore, prevention of PHS or minimizing its negative effects in wheat requires the development of wheat cultivars with moderate level of dormancy in the seeds. However, the development of such cultivars requires the need to identify genomic regions and genes that are responsible for PHS resistance. Therefore, this research was focused on genome wide association study of a collection of spring wheat lines, which show a wide range of variation in seed dormancy, over six trial environments. Analysis of the association between the phenotypic and genotypic data of wheat lines studied led to the detection of the genomic regions and candidate genes that might have the potential to introduce PHS resistance in spring wheat.