Innovative approaches to assessing seed quality in Brassicas

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dc.contributor.authorLarkin, Jodi
dc.contributor.examiningcommitteeLi, Genyi (Plant Science) Belmonte, Mark (Biological Sciences)en_US
dc.contributor.supervisorMcVetty, Peter (Plant Science)en_US
dc.date.accessioned2012-04-02T20:24:32Z
dc.date.available2012-04-02T20:24:32Z
dc.date.issued2012-04-02
dc.degree.disciplinePlant Scienceen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractBrassica napus is grown as an edible oil (canola) and an industrial oil (HEAR). Its fatty acid profile and chlorophyll concentration affect the quality. It is important to develop accurate and efficient methods to evaluate these traits. The first objective of this study was to improve the single nucleotide polymorphism (SNP) and sequence characterized amplified region (SCAR) molecular markers for erucic acid genotypes in the Bn-FAE1.1 and Bn-FAE1.2 genes in the A and C genomes in Brassica napus originally developed by Rahman et al. (2008). When put into practice, the error rate was unacceptably high. With the modifications that were made to the protocols, the overall accuracy remained relatively consistent indicating that further improvements are still required. The second objective was to develop a near infrared reflectance (NIR) based calibration equation for chlorophyll concentration in whole Brassica napus seeds. In this case, an equation was successfully created.en_US
dc.description.noteMay 2012en_US
dc.identifier.urihttp://hdl.handle.net/1993/5230
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectBrassica napusen_US
dc.subjectchlorophyll concentrationen_US
dc.subjecterucic acid genesen_US
dc.subjectnear infrared reflectance spectroscopyen_US
dc.subjectmolecular markersen_US
dc.titleInnovative approaches to assessing seed quality in Brassicasen_US
dc.typemaster thesisen_US
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