Global RNA profiling of susceptible and tolerant genotypes of Brassica napus infected with Sclerotinia sclerotiorum and prediction and functional characterization of novel regulators of plant defense

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dc.contributor.authorGirard, Ian
dc.contributor.examiningcommitteeBelmonte, Mark (Biological Sciences) Fernando, Dilantha (Plant Sciences) Chen, Guanqun (Biological Sciences)en_US
dc.contributor.supervisorBelmonte, Mark (Biological Sciences) Fernando, Dilantha (Plant Sciences)en_US
dc.date.accessioned2016-09-27T14:24:37Z
dc.date.available2016-09-27T14:24:37Z
dc.date.issued2016-05-31en_US
dc.date.issued2016en_US
dc.degree.disciplineBiological Sciencesen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractBrassica napus (L.) contributes over $19 billion dollars each year to the Canadian economy. However, yields are constantly threatened by Sclerotinia sclerotiorum (Lib) de Bary, the fungus responsible for Sclerotinia stem rot. To date, there are no global RNA profiling data or gene regulatory analyses of plant tissues directly at the main site of foliar infection in the B. napus-S. sclerotiorum pathosystem. Using RNA sequencing and a gene regulatory analysis, I discovered putative transcriptional regulators of biological processes associated with the tolerant phenotype of B. napus cv. Zhougyou821 including subcellular localization of proteins, pathogen detection, and redox homeostasis. Functional characterization of Arabidopsis mutants identified a number of genes that contribute directly to plant defense to S. sclerotiorum. Together this research amounts to the expansion of our understanding of the B. napus-S. sclerotiorum pathosystem and a valuable resource to help protect B. napus crops from virulent pathogens such as S. sclerotiorum.en_US
dc.description.noteOctober 2016en_US
dc.identifier.citationGirard, I. J., Mcloughlin, A. G., de Kievit, T. R., Fernando, D. W. G., & Belmonte, M. F (2016). Integrating Large-Scale Data and RNA Technology to Protect Crops from Fungal Pathogens. Frontiers in Plant Science, 7, 1-7. doi: 10.3389/fpls.2016.00631en_US
dc.identifier.citationGirard, I. J., Tong, C., Mao, X., Becker, M. G., Huang, J., de Kievit, T. R., Fernando, D. W. G., Liu, S., & Belmonte, M. F. (2016). RNA profiling of the Sclerotinia sclerotiorum-Brassica napus pathosystem reveals crosstalk between redox and hormone pathways. Molecular Plant, submitted: June 30th 2016en_US
dc.identifier.urihttp://hdl.handle.net/1993/31862
dc.language.isoengen_US
dc.publisherFrontiersen_US
dc.publisherMolecular Planten_US
dc.rightsopen accessen_US
dc.subjectRNA sequencingen_US
dc.subjectPlant-pathogen interactionsen_US
dc.titleGlobal RNA profiling of susceptible and tolerant genotypes of Brassica napus infected with Sclerotinia sclerotiorum and prediction and functional characterization of novel regulators of plant defenseen_US
dc.typemaster thesisen_US
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