Characterization of 3 different types of aquaporins in Carcinus maenas and their potential role in osmoregulation

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dc.contributor.authorNash, Mikyla
dc.contributor.examiningcommitteeJeffries, Kennethen_US
dc.contributor.examiningcommitteeTreberg, Jasonen_US
dc.contributor.supervisorWeihrauch, Dirk
dc.date.accessioned2022-08-12T16:59:36Z
dc.date.available2022-08-12T16:59:36Z
dc.date.copyright2022-08-12
dc.date.issued2022-08-02
dc.date.submitted2022-08-12T16:25:48Zen_US
dc.degree.disciplineBiological Sciencesen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractIntertidal crustaceans like Carcinus maenas shift between an osmoconforming and osmoregulating state when inhabiting full-strength seawater and dilute environments, respectively. While the bodily fluids and environment of marine osmoconformers are approximately isosmotic, osmoregulating crabs inhabiting dilute environments maintain their bodily fluid osmolality above that of their environment by actively absorbing and retaining osmolytes (e.g., Na+, Cl-, urea) while eliminating excess water. Few studies have investigated the role of aquaporins (AQPs) in the osmoregulatory organs of crustaceans, especially within brachyuran species. In the current study, three different aquaporins were identified within a transcriptome of C. maenas, including a classical AQP (CmAQP1), an aquaglyceroporin (CmGLP1), and a big-brain protein (CmBIB1), all of which are expressed in the gills and the antennal glands. Functional expression of these aquaporins confirmed water transport capabilities for CmAQP1, CmGLP1, but not for CmBIB1, while CmGLP1 also transported urea. Higher relative CmAQP1 mRNA expression within tissues of osmoconforming crabs suggests the apical/sub-apically localized channel attenuates osmotic gradients created by non- osmoregulatory processes while its downregulation in dilute media reduces the water permeability of tissues to facilitate osmoregulation. Although hemolymph urea concentrations rose upon exposure to brackish water, urea was not detected in the final urine. Due to its urea- transport capabilities, CmGLP1 is hypothesized to be involved in a urea retention mechanism believed to be involved in the production of diluted urine. Overall, these results suggest that AQPs are involved in osmoregulation and provide a basis for future mechanistic studies investigating the role of AQPs in volume regulation in crustaceans.en_US
dc.description.noteOctober 2022en_US
dc.description.sponsorshipUniversity of Manitoba Graduate Enhancement of Tri-agency Stipends Granten_US
dc.identifier.urihttp://hdl.handle.net/1993/36679
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectUrineen_US
dc.subjectWater transporten_US
dc.subjectUrea transporten_US
dc.subjectCrustaceansen_US
dc.subjectGillsen_US
dc.subjectBrainen_US
dc.subjectQuantitative PCRen_US
dc.subjectHeterologous protein expressionen_US
dc.subjectAntennal glandsen_US
dc.titleCharacterization of 3 different types of aquaporins in Carcinus maenas and their potential role in osmoregulationen_US
dc.typemaster thesisen_US
local.subject.manitobanoen_US
oaire.awardNumberRGPIN/5013- 2018en_US
oaire.awardTitleNSERC Discovery Granten_US
project.funder.identifierhttps://doi.org/10.13039/501100000038en_US
project.funder.nameNational Science and Engineering Research Council of Canada (NSERC)en_US
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