Investigation of T Cell Chemotaxis and Electrotaxis Using Microfluidic Devices

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dc.contributor.authorLi, Jing
dc.contributor.examiningcommitteeHu, Can-Ming (Physics and Astronomy) Major, Arkady (Electrical and Computer Engineering)en_US
dc.contributor.supervisorLin, Francis (Physics and Astronomy)en_US
dc.date.accessioned2012-06-22T19:37:12Z
dc.date.available2012-06-22T19:37:12Z
dc.date.issued2011en_US
dc.date.issued2011en_US
dc.date.issued2012en_US
dc.degree.disciplinePhysics and Astronomyen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractDirected immune cell migration plays important roles in immunosurveillance and immune responses. Understanding the mechanisms of immune cell migration is important for the biology of immune cells with high relevance to immune cell trafficking mediated physiological processes and diseases. Immune cell migration can be directed by various guiding cues such as chemical concentration gradients (a process termed chemotaxis) and direct current electric fields (dcEF)(a process termed electrotaxis). Microfluidic devices that consist of small channels with micrometer dimensions have been increasingly developed for cell migration studies. These devices can precisely configure and flexibly manipulate chemical concentration gradients and electric fields, and thus provide powerful quantitative test beds for studying the complex guiding mechanisms for cell migration. In the research of this thesis, a PDMS-based and a glass-based microfluidic devices were developed for producing controlled dcEF and these devices were used to analyze electrotaxis of activated human blood T cells. Using both devices, we have successfully demonstrated that activated human blood T cells migrate toward the cathode of the applied dcEF. Furthermore, a novel microfluidic device was developed to configure better controlled single or co-existing chemical gradients and dcEF to mimic the complex guiding environments in tissues and this device was used to investigate the competition of chemical gradients and dcEF in directing activated human blood T cell migration.en_US
dc.description.noteOctober 2012en_US
dc.identifier.citationJ. Li, S. Nandagopal, D. Wu, S.F. Romanuik, K. Paul, D.J. Thomson and F. Lin, "Activated T Lymphocytes Migrate Toward the Cathode of DC Electric Fields in Microfluidic Devices", Lab on a Chip, 2011, 11(7), 1298 -1304.en_US
dc.identifier.citationJ. Li and F. Lin, "Microfluidic Devices for Studying Chemotaxis and Electrotaxis", Trends in Cell Biology, 2011, Vol. 21, 489-497.en_US
dc.identifier.citationJ. Li, L. Zhu, M. Zhang and F. Lin, "Microfluidic Device for Studying Cell Migration in Single or Co-Existing Chemical Gradients and Electric Fields", Biomicrofluidics, 2012, 6, 024121en_US
dc.identifier.urihttp://hdl.handle.net/1993/8093
dc.language.isoengen_US
dc.publisherRoyal Society of Chemistry (RSC)en_US
dc.publisherElsevieren_US
dc.publisherAmerican Institute of Physicsen_US
dc.rightsopen accessen_US
dc.subjectCell migrationen_US
dc.subjectelectrotaxisen_US
dc.subjectchemotaxisen_US
dc.subjectmicrofluidicsen_US
dc.titleInvestigation of T Cell Chemotaxis and Electrotaxis Using Microfluidic Devicesen_US
dc.typemaster thesisen_US
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