FTIR spectrochemical imaging analysis of lipid and protein content of bottom-ice diatoms along a tidal strait within the Kitikmeot Sea, Canadian Arctic

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dc.contributor.authorPogorzelec, Nicole
dc.contributor.examiningcommitteeWalkusz, Wojciech (Environment and Geography)en_US
dc.contributor.examiningcommitteeMorrison, Jason (Biosystems Engineering)en_US
dc.contributor.supervisorMundy, C.J. (Environment and Geography) Gough, Kathleen (Chemistry)en_US
dc.date.accessioned2020-01-14T15:53:37Z
dc.date.available2020-01-14T15:53:37Z
dc.date.issued2019en_US
dc.date.submitted2019-12-20T23:44:46Zen
dc.degree.disciplineEnvironment and Geographyen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractWithin the ice bottom, ice algae begin their annual Arctic spring bloom in which they contribute up to half of the total annual primary production in the central Arctic Ocean. Essential fats (or lipids) are produced during the early portion of the ice algal bloom (e.g., polyunsaturated fatty acids (PUFAs) such as omega-3 fatty acids) and are of particular importance to the energy-rich Arctic marine ecosystem. Fourier Transform Infrared (FTIR) Spectrochemical imaging is an efficient and non-destructive analytical technique, which allows us to measure changes in ice algal biomolecular composition (i.e. saturated lipid, protein and biogenic silica). In this thesis, we examine diatom samples collected from first-year landfast sea ice across a tidal strait region located between the Finlayson Islands within Dease Strait of the Kitikmeot Sea, near Cambridge Bay, Nunavut, Canada. This research was conducted under the ICE-CAMPS (Ice Covered Ecosystem-Cambridge Bay Process Study) 2017 spring field campaign. It was determined, via two case studies: i. the relative amount of biomolecular composition was a function of cell size and species. ii. individual cell biomolecular composition trends were not depicted in bulk measurements (i.e. particulate organic carbon, chlorophyll a, etc.) iii. Two expected nutrient gradients were observed within the tidal strait region, one spatially and one vertically iv. lipid (protein) content increased (decreased) spatially in response to nutrient limitation, while lipid (protein) content increased (remained constant) vertically in the sea-ice v. the creation of new FTIR imaging methods were successful in rapidly imaging the diatom community. The findings presented here have reinforced the usefulness of the FTIR instrument to diatom autecological analysis. This sensitive, non-destructive and semi-quantitative analytical technique has allowed us to further our understanding not only of the ice algal community, but also the autecology of individual diatom taxa.en_US
dc.description.noteFebruary 2020en_US
dc.identifier.urihttp://hdl.handle.net/1993/34505
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectFTIRen_US
dc.subjectDiatomen_US
dc.subjectAttheya spp.en_US
dc.subjectNitzschia frigidaen_US
dc.subjectProteinen_US
dc.subjectLipiden_US
dc.subjectBimolecular Compositionen_US
dc.subjectBiomassen_US
dc.subjectArcticen_US
dc.subjectTidal Straiten_US
dc.titleFTIR spectrochemical imaging analysis of lipid and protein content of bottom-ice diatoms along a tidal strait within the Kitikmeot Sea, Canadian Arcticen_US
dc.typemaster thesisen_US
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