Reduced catabolic protein expression in

Gungormusler-Yilmaz, Mine; Shamshurin, Dmitry; Grigoryan, Marine; Taillefer, Marcel; Spicer, Victor; Krokhin, Oleg V; Sparling, Richard; Levin, David B

Reduced catabolic protein expression in

dc.contributor.author	Gungormusler-Yilmaz, Mine
dc.contributor.author	Shamshurin, Dmitry
dc.contributor.author	Grigoryan, Marine
dc.contributor.author	Taillefer, Marcel
dc.contributor.author	Spicer, Victor
dc.contributor.author	Krokhin, Oleg V
dc.contributor.author	Sparling, Richard
dc.contributor.author	Levin, David B
dc.date.accessioned	2014-09-01T15:25:36Z
dc.date.available	2014-09-01T15:25:36Z
dc.date.issued	2014-08-30
dc.date.updated	2014-09-01T15:25:39Z
dc.description.abstract	Abstract Higher initial glycerol loadings (620 mM) have a negative effect on growth and 1,3-propanediol (1,3-PDO) synthesis in Clostridium butyricum DSM 10702 relative to lower initial glycerol concentrations (170 mM). To help understand metabolic shifts associated with elevated glycerol, protein expression levels were quantified by LC/MS/MS analyses. Thirty one (31) proteins involved in conversion of glycerol to 1,3-PDO and other by-products were analyzed by multiple reaction monitoring (MRM). The analyses revealed that high glycerol concentrations reduced cell growth. The expression levels of most proteins in glycerol catabolism pathways were down-regulated, consistent with the slower growth rates observed. However, at high initial glycerol concentrations, some of the proteins involved in the butyrate synthesis pathways such as a putative ethanol dehydrogenase (CBY_3753) and a 3-hydroxybutyryl-CoA dehydrogenase (CBY_3045) were up-regulated in both exponential and stationary growth phases. Expression levels of proteins (CBY_0500, CBY_0501 and CBY_0502) involved in the reductive pathway of glycerol to 1,3-PDO were consistent with glycerol consumption and product concentrations observed during fermentation at both glycerol concentrations, and the molar yields of 1,3-PDO were similar in both cultures. This is the first report that correlates expression levels of glycerol catabolism enzymes with synthesis of 1,3-PDO in C. butyricum. The results revealed that significant differences in the expression of a small subset of proteins were observed between exponential and stationary growth phases at both low and high glycerol concentrations.
dc.description.version	Peer Reviewed
dc.identifier.citation	AMB Express. 2014 Aug 30;4(1):63
dc.identifier.doi	http://dx.doi.org/10.1186/s13568-014-0063-6
dc.identifier.uri	http://hdl.handle.net/1993/23921
dc.language.rfc3066	en
dc.rights	open access	en_US
dc.rights.holder	Mine Gungormusler-Yilmaz et al.; licensee BioMed Central Ltd.
dc.title	Reduced catabolic protein expression in
dc.type	Journal Article