25 Hydroxycholesterol inhibits adipogenesis and expression of adipogenic transcripts in C3H10T1/2 mouse stem cells independent of hedgehog signalling mechanism

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dc.contributor.authorMoseti, Dorothy
dc.contributor.examiningcommitteeO, Karmin (Animal Science) Anderson, Judith (Biological Sciences)en_US
dc.contributor.supervisorkim, Woo-kyun (Animal Science)en_US
dc.date.accessioned2015-06-15T19:43:47Z
dc.date.available2015-06-15T19:43:47Z
dc.date.issued2015-06-15
dc.degree.disciplineAnimal Scienceen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractThis study was conducted to assess the effects of specific oxysterols on the adipogenic differentiation and expression of adipogenic transcripts in C3H10T1/2 mouse stem cells. In the first study, four oxysterols namely; 20S, 22R, 22S and 25 hydroxycholesterol (25-HC) were tested to determine which one best inhibits adipogenesis in C3H10T1/2 mouse stem cells. Adipogenic differentiation was induced using an adipogenic media (DMITro) consisting of dexamethasone (DEX), 3-isobutyl-1-methyl-xanthine (IBMX), insulin and troglitazone (Tro). Inhibition of adipogenesis was assessed by treatment of cells with DMITro+20S, 22R, 22S or 25-HC for six days. Oil red O pictures and gene expression analysis showed that 25-HC was more effective in inhibiting the expression of adipogenic genes compared to the other oxysterols. Further investigation of the mechanisms of action of 25-HC showed that the inhibitory effects of 25-HC on adipogenesis are not mediated by hedgehog signalling.en_US
dc.description.noteOctober 2015en_US
dc.identifier.urihttp://hdl.handle.net/1993/30577
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectAdipogenesisen_US
dc.subjectOxysterolsen_US
dc.subjectC3H10T1/2 stem cellsen_US
dc.title25 Hydroxycholesterol inhibits adipogenesis and expression of adipogenic transcripts in C3H10T1/2 mouse stem cells independent of hedgehog signalling mechanismen_US
dc.typemaster thesisen_US
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