A forward genetic screen for Caenorhabditis elegans pharyngeal gland cell defects and characterization of mutagenized strains with a homozygous viable gland cell under migration

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dc.contributor.authorTkachuk, Stephanie
dc.contributor.examiningcommitteeMarcus, Jeffrey (Biological Sciences) Merz, David (Biochemistry and Medical Genetics)en_US
dc.contributor.supervisorKormish, Jay (Biological Sciences)en_US
dc.date.accessioned2018-04-12T19:23:32Z
dc.date.available2018-04-12T19:23:32Z
dc.date.issued2018
dc.date.submitted2018-04-02T15:13:30Zen
dc.degree.disciplineBiological Sciencesen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractCaenorhabditis elegans is a powerful genetic tool to study development. The pharynx is a neuromuscular organ of the upper digestive tract used to study how cells regulate their shape and movements during development. In the embryo, a receptor tyrosine kinase-like receptor (ROR), cam-1, is necessary for a single dorsal gland cell to migrate through the pharynx. A mutagenesis screen to isolate factors that may work with cam-1 examined 4,986 haploid genomes and isolated 60 strains with gland cell defects. Snip-SNP mapping placed one strain on the first chromosome, seven strains on the fourth, and one strain on the X chromosome. Complementation crosses and whole genome sequencing identified the LG IV mutations as cwn-2, a Wnt ligand, and ham-1, a storkhead box factor. The mutations on LG I and X require further testing to confirm the causative lesion. ham-1 RNAi in the cam-1 null background suggests that ham-1 and cam-1 function in different genetic pathways.en_US
dc.description.noteMay 2018en_US
dc.identifier.urihttp://hdl.handle.net/1993/32969
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectDevelopmental biologyen_US
dc.subjectCaenorhabditis elegansen_US
dc.subjectOrgan developmenten_US
dc.subjectPharynx foreguten_US
dc.subjectGland cellsen_US
dc.subjectMolecular geneticsen_US
dc.titleA forward genetic screen for Caenorhabditis elegans pharyngeal gland cell defects and characterization of mutagenized strains with a homozygous viable gland cell under migrationen_US
dc.typemaster thesisen_US
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