Age-related Changes in p56lck Protein Levels and Phenotypic Distribution of T Lymphocytes in Young Rats

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dc.contributor.authorHosea, Heather J.
dc.contributor.authorRector, Edward S.
dc.contributor.authorTaylor, Carla G.
dc.date.accessioned2014-08-14T07:06:37Z
dc.date.available2014-08-14T07:06:37Z
dc.date.issued2005-03
dc.date.updated2014-08-14T07:06:37Z
dc.description.abstractp56lck is involved in the maturation of T-cells from double negative (DN) into double positive (DP) T-cells. The objective of this experiment was to determine changes in the levels of thymic and splenic T-cell p56lck using Western immunoblotting, along with the proportion and number of T-cell subsets in thymus, spleen and blood using flow cytometry in growing Sprague-Dawley rats. Thymic p56lck levels were negatively correlated with age (r=-0.42, p=0.04) and positively correlated with age in the spleen (r=0.50, p=0.01). Nine-week-old rats had a higher percentage of thymic DN and CD8 cells with fewer DP cells compared to younger rats. There were minor differences in the proportions of T-cell subsets in the spleen and blood. T-cell numbers remained proportional to body weight in the lymphoid organs; however, the lower absolute number of T-cells in the younger rats might indicate that they are less able to respond to antigens.
dc.description.versionPeer Reviewed
dc.identifier.citationHeather J. Hosea, Edward S. Rector, and Carla G. Taylor, “Age-related Changes in p56lck Protein Levels and Phenotypic Distribution of T Lymphocytes in Young Rats,” Clinical and Developmental Immunology, vol. 12, no. 1, pp. 75-84, 2005. doi:10.1080/17402520400017393
dc.identifier.doihttp://dx.doi.org/10.1080/17402520400017393
dc.identifier.urihttp://hdl.handle.net/1993/23785
dc.language.rfc3066en
dc.rightsopen accessen_US
dc.rights.holderCopyright © 2005 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.titleAge-related Changes in p56lck Protein Levels and Phenotypic Distribution of T Lymphocytes in Young Rats
dc.typejournal articleen_US
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