Anti-inflammatory role of chromofungin, a chromogranin A-derived peptide, in the context of colitis
Loading...
Date
2018
Authors
Kapoor, Kunal
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Ulcerative colitis(UC) is characterized by a decreased level of chromofungin (CHR: Chormogranin-A 47-66) and a dysregulation of CD11c+ cells. We aimed to investigate the association between CHR and CD11c+ cells in an experimental model of UC. Colitis was induced in C57BL/6 mice by 5% dextran sulfate sodium with intra-rectal injection of CHR. Disease activity was monitored and colonic, mesenteric lymph node and splenic levels of pro-inflammatory cytokines and CD markers were assessed using ELISA and/or RT-qPCR. In-vivo, CHR treatment reduced colitis with a significant decrease in colonic levels of pro-inflammatory markers. In MLN, CHR decreased CD11c+ markers and in splenic CD11c+ cells CHR decreased CD40 and CD80. RT-2 PCR array demonstrated a downregulation of NF-kB in colon and in CD11c+ isolated cells from mice treated with CHR. NF-kB blocker/activator studies confirmed regulation by CHR. CHR regulates the development of colitis through the modulation of CD11c+ cells markers of immune activation.
Description
Keywords
Ulcerative Colitis