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    Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species

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    13567_2018_Article_605.pdf (1.307Mb)
    Date
    2018-10-29
    Author
    Beninger, Caroline
    Naqvi, Syed A
    Naushad, Sohail
    Orsel, Karin
    Luby, Chris
    Derakhshani, Hooman
    Khafipour, Ehsan
    De Buck, Jeroen
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    Abstract
    Abstract Digital dermatitis (DD) presents as painful, ulcerative or proliferative lesions that lead to bovine lameness affecting economic efficiency and animal welfare. Although DD etiological agent(s) have not been established, it is widely accepted that DD is a polymicrobial disease significantly associated with species of Treponema and the non-linear disease progression may be attributed to interactions among infecting bacteria. We postulated the morphological changes associated with DD lesion grades are related to interactions among infecting species of Treponema. We developed a novel species-specific qPCR that can identify the absolute abundance of the four of the most common species of Treponema in DD, T. phagedenis, T. medium, T. pedis and T. denticola, in a single reaction. We found species abundance and the number of distinct Treponema species present is higher in active, ulcerative lesions than in healing lesions, chronic lesions, and DD-free skin. Treponema spp. were present in both DD-free skin and M3 lesions following treatment with oxytetracycline. We have also found positive correlations among T. phagedenis, T. medium and T. pedis indicating they are significantly more likely to be found together than apart and their absolute quantities tend to increase together, a relationship which is not present with T. denticola. Further, we found Treponema, particularly viable T. denticola, in lesions 5 days post treatment with oxytetracycline (M3). Our findings suggest that pathogenicity may be closely associated with Treponema abundance, particularly T. phagedenis, T. medium and T. pedis, and interactions among them, independent of T. denticola. Our results provide a novel, consistent method to identify species of Treponema within DD lesions and associate Treponema spp. and abundance with morphological changes related to host pathogenicity.
    URI
    https://doi.org/10.1186/s13567-018-0605-z
    http://hdl.handle.net/1993/33548
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    • Faculty of Agricultural and Food Sciences Scholarly Works [97]
    • University of Manitoba Scholarship [1978]

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