Development and Characterization of Magnetic Nanoparticles for Treating Glioblastoma
Loading...
Date
2018-08-23
Authors
Kim, Suhyun
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Glioblastoma multiforme (GB) is the most common and most aggressive brain cancer in adults with a median survival time after diagnosis of 12 months even with multimodal treatments. Chemotherapeutics have limited response in GB due to inadequate drug accumulation at the tumor site. In response to limited chemotherapeutic options for treating GB, many researchers are investigating the use of nanoparticles (NPs), synthetically engineered particulates that are < 100 nm in size, as a novel drug
delivery mechanism. The present study examined two different non-spherical magnetic nanoparticles in an effort to understand the mechanisms governing nanoparticle accumulation, cellular drug delivery and cytotoxicity in established human GB cell lines. The hypothesis of these studies is that nanoparticle accumulation occurs through specific endocytic pathways that can be altered by application of external magnetic fields. Two non-spherical shaped iron oxide nanoparticles (IONPs), iron oxide nanodiscs
(IONDs) and iron oxide nanobricks (IONBs) with identical surface coating of N-(trimethoxysilylpropyl)
ethylenediaminetriacetate trisodium salt (EDT) were evaluated for cellular accumulation, drug delivery and cytotoxic response profiles using established human GB cell lines. Both IONP compositions showed both temperature-dependent and magnetic field-dependent cellular accumulation. Cell accumulation appeared to be through a caveolin-based endocytic pathway. Doxorubicin (DOX) loading onto the IONP was greatest for EDT-IONB composition and these nanoparticles were further shown to produce cytoxic
responses in GB cells. Based on these studies, EDT-IONB is a reasonable nanoparticle composition to advance for further in vivo studies for glioblastoma treatment.
Description
Keywords
Glioblastoma multiforme (GB), EDT-IONB, nanoparticles (NPs), iron oxide nanoparticles (IONPs)
Citation
AMA