Quantitative Trait Loci Controlling Sclerotinia Stem Rot Resistance and Seed Glucosinolate Content of Oilseed Rape (Brassica napus L.)

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dc.contributor.supervisor Li, Genyi (Plant Science) en_US
dc.contributor.author Liu, Jun
dc.date.accessioned 2016-09-09T17:36:15Z
dc.date.available 2016-09-09T17:36:15Z
dc.date.issued 2016 en_US
dc.identifier.uri http://hdl.handle.net/1993/31662
dc.description.abstract Canola/rapeseed (Brassica napus L.) is a major oilseed crop worldwide. However, its production is largely affected by the fungal disease Sclerotinia stem rot as well as seed glucosinolates. So far the genetic mechanisms controlling these two traits have been poorly understood. In the present study, three bi-parental doubled haploid B. napus populations M730, M692 and ZT were grown in either natural or artificial environments and genotyped using the Brassica 60K Infinium® SNPs and/or sequence related amplified polymorphisms. Three genetic linkage maps covered 2,597.7 cM, 2,474.1 cM and 1,731.6 cM in 19 chromosomes for M730, M692 and ZT, respectively. Plants were inoculated with Sclerotinia sclerotiorum mycelia on stems at the reproductive stage to evaluate their resistivity. Four aliphatic glucosinolates and one indolic glucosinolate were detected in the seeds using high-performance liquid chromatography. 4-hydroxy-3-indolylmethyl predominated over aliphatic glucosinolates in canola, but inversely constituted a small portion of total glucosinolate content in semi-winter rapeseed. In rapeseed, 2-hydroxy-3-butenyl predominated in 4C aliphatic glucosinolates, which in turn predominated in total aliphatic glucosinolates, which likewise predominated in total glucosinolate content. QTLs regulating major glucosinolates were located on chromosome A9 for high glucosinolate content populations M730 and ZT, and on chromosome C7 for low glucosinolate content population M692. Major QTLs for Sclerotinia stem rot resistance were located on chromosomes A7 and C6 in M730, on chromosomes A3 and A7 in ZT, while no major QTLs were found in M692. Additive genetic effect was the major factor explaining phenotypic variations of the two traits. No direct genetic relationship was observed between Sclerotinia stem rot resistance in adult plants and seed glucosinolates in B. napus. The findings in the studies could be used to formulate breeding and research strategies in B. napus and the major QTLs controlling the two traits and their closely linked SNP markers could be validated over wide germplasm and used in marker assisted selection. en_US
dc.publisher Australian Journal of Plant Science en_US
dc.rights info:eu-repo/semantics/openAccess
dc.subject Brassica napus en_US
dc.subject Sclerotinia stem rot en_US
dc.subject glucosinolate en_US
dc.subject Quantitative trait loci en_US
dc.subject Sclerotinia sclerotiorum en_US
dc.title Quantitative Trait Loci Controlling Sclerotinia Stem Rot Resistance and Seed Glucosinolate Content of Oilseed Rape (Brassica napus L.) en_US
dc.type info:eu-repo/semantics/doctoralThesis
dc.degree.discipline Plant Science en_US
dc.contributor.examiningcommittee McVetty, Peter (Plant Science) Belmonte, Mark (Biological Sciences) Wu, Chunren (Plant Science) Li, Genyi (Plant Science) Weselake, Randall (University of Alberta) en_US
dc.degree.level Doctor of Philosophy (Ph.D.) en_US
dc.description.note October 2016 en_US

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