A Preliminary Guideline for the Assignment of Methicillin-Resistant Staphylococcus aureus to a Canadian Pulsed-Field Gel Electrophoresis Epidemic Type Using spa Typing
Golding, George R
Campbell, Jennifer L
Spreitzer, Dave J
Mulvey, Michael R
Canadian Nosocomial Infection Surveillance Program,
BACKGROUND: Increasing rates of methicillin-resistant Staphylococcus aureus (MRSA) infections on a global scale is a major health concern. In Canada, there are 10 known epidemic types of MRSA as determined by pulsed-field gel electrophoresis (PFGE). Despite the excellent discriminatory power of PFGE, there are several disadvantages of using this technique, such as high degree of labour intensity and the inability to easily develop an MRSA typing database due to the subjective interpretation of results.OBJECTIVES: The purpose of the present study was to determine whether spa typing, an established DNA sequence-based typing method, could be used as an alternative to PFGE for the typing of Canadian MRSA (CMRSA) epidemic isolates.RESULTS: spa types were determined for 1488 CMRSA isolates, and the method was analyzed for its ability to identify and cluster CMRSA1-10 strains. Minimal spanning tree analysis of 1452 spa types revealed individual clonal clusters for PFGE epidemic types CMRSA1, 2, 7 and 8, but spa typing could not distinguish CMRSA5 from CMRSA9 and CMRSA10, and CMRSA3 from CMRSA4 and CMRSA6. However, specific spa types were generally associated with only one PFGE epidemic type. Based on these results, a spa typing guideline for CMRSA isolates was developed and tested using the first 300 MRSA isolates received in 2007 through the Canadian Nosocomial Infection Surveillance Program.CONCLUSIONS: The high concordance of spa types with PFGE epidemic types using this guideline demonstrated the feasibility of spa typing as a more rapid and less technically demanding alternative typing method for MRSA in Canada.
George R Golding, Jennifer L Campbell, Dave J Spreitzer, et al., “A Preliminary Guideline for the Assignment of Methicillin-Resistant Staphylococcus aureus to a Canadian Pulsed-Field Gel Electrophoresis Epidemic Type Using spa Typing,” Canadian Journal of Infectious Diseases and Medical Microbiology, vol. 19, no. 4, pp. 273-281, 2008. doi:10.1155/2008/754249