Multiple environmental factors regulate the expression of the carbohydrate-selective OprB porin of Pseudomonas aeruginosa
Loading...
Date
1999-12-31
Authors
Adewoye, LO
Worobec, EA
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
In response to low extracellular glucose concentration, Pseudomonas aeruginosa induces the expression of the outer membrane carbohydrate-selective OprB porin. The promoter region of the oprB gene was cloned into a lacZ transcriptional fusion vector, and the construct was mobilized into P. aeruginosa OprB-deficient strain, WW100, to evaluate additional environmental factors that influence OprB porin gene expression. Growth temperature, pH of the growth medium, salicylate concentration, and carbohydrate source were found to differentially influence porin expression. This expression pattern was compared to those of whole-cell [C-14]glucose uptake under conditions of high osmolarity, ionicity, variable pH, growth temperatures, and carbohydrate source. These studies revealed that the high-affinity glucose transport genes are down-regulated by salicylic acid, differentially regulated by pH and temperature, and are specifically responsive to exogenous glucose induction.
Description
Keywords
Pseudomonas aeruginosa, OprB porin, glucose transport, regulation, ESCHERICHIA-COLI K-12, OUTER-MEMBRANE PROTEIN, GENE-EXPRESSION, TRANSPORT-SYSTEM, CHANNEL SIZE, SIGNAL TRANSDUCTION, OSMOTIC REGULATION, MOLECULAR ANALYSIS, CYSTIC-FIBROSIS, PH
Citation
0008-4166; CAN J MICROBIOL, DEC 1999, vol. 45, no. 12, p.1033 to 1042.