Modulation of TGF-B in platelets and neutrophils by dietary lipids
Adam, Jay M.
Dietary lipids have been implicated in the pathology and prevention of variety of chronic illnesses including cancer and atherosclerosis. Transforming growth factor-beta (TGF-$\beta)$ is a multifunctional cytokine that is believed to modulate the key steps in pathology of these chronic illnesses. Platelets and neutrophils are known to play a role in the development of these illnesses and are also known to transport high concentrations of TGF-$\beta$ throughout the vascular system. Therefore the possibility that dietary lipids modulate the TGF-$\beta$ content of these cells was investigated in a series of studies. A preliminary study was conducted with weanling Spragure-Dawley rats to assess TGF-$\beta$ status of platelets as affected by dietary lipids utilising the CCL-64 bioassay. The growth inhibitory effect of platelet lysate, employing the CCL-64 bioassay, was quantified to reflect TGF-$\beta$ content. Rats were put on four different high fat diets and one low fat diet for 6 weeks. Diets were composed of 18% test fat and 5% soy oil, by weight. Beef tallow (HFB), fish oil (HFF), corn oil (HFC), and olive oil (HFO) were used for the high fat diets and a low fat soy oil (LFS) diet was included with 5% soy oil. Supplementary studies were conducted with the neutrophils and plasma of the older rats. (Abstract shortened by UMI.)