Effects of dietary zinc deficiency and malnutrition on the T-lymphocyte zinc-finger protein p56[1]c[superscript]k[superscript] in mice

Thumbnail Image
Lepage, Lynne Marie Gisele
Journal Title
Journal ISSN
Volume Title
The objective of this experiment was to investigate the effects of dietary zinc deficiency (ZnDF) and malnutrition syndromes (2% protein deficiency (LP), combined Zn and 2% protein deficiencies (ZnDF + LP) and energy restriction (ER)) compared to the control group (C), on the type of malnutrition, zinc status, splenocyte counts and T-Lymphocyte expression of p56$\rm \sp{lck}$, an early T-lymphocyte signal transduction Zn-finger protein. An adult murine model of zinc deficiency and malnutrition syndromes was developed in order to investigate these parameters. Following a 4 wk feeding trial, mice receiving the ZnDF + LP, ZnDF, ER, and LP diets weighed significantly less than the C group, however, different forms of malnutrition were induced. Protein-type malnutrition was induced in the ZnDF + LP and LP groups, and energy-type malnutrition was induced in the ZnDF and ER groups. Significant decreases in serum zinc and femur zinc pools were observed in the ZnDF + LP and ZnDF groups. A reduction in splenocyte counts was observed in the ZnDF groups. $(1.56 \pm 0.06 \times 10\sp8)$ compared to the C group $(1.91 \pm 0.13 \times 10\sp8),$ however, the ER group $(1.44 \pm 0.03 \times 10\sp8)$ experienced a decrease similar to that of the ZnDF group. The expression of p56$\rm\sp{lck}$ was elevated in all treatment groups compared with the control group. Surprisingly, the ZnDF + LP and the ZnDF groups had approximately a 1.7 fold increase in p56$\sp{\rm lck}$ expression compared to the control group. Thus, it appears that dietary Zn deficiency may play a role in up-regulating the expression of p56$\rm\sp{lck}.$ (Abstract shortened by UMI.)