The Molecular Characterization and The Generation of a Reverse Genetics System for Kyasanur Forest Disease Virus

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dc.contributor.authorCook, Bradley William Michael
dc.contributor.examiningcommitteede Kievit, Teresa (Microbiology) Levin, David (Biosystems Engineering) Theriault, Steven (Microbiology)en
dc.contributor.supervisorCourt, Deborah A. (Microbiology) Theriault, Steven (Microbiology)en
dc.date.accessioned2010-04-09T17:26:06Z
dc.date.available2010-04-09T17:26:06Z
dc.date.issued2010-04-09T17:26:06Z
dc.degree.disciplineMicrobiologyen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractKyasanur Forest Disease Virus (KFDV) is a tick-borne, hemorrhagic fever-causing member of the Flaviviridae. With infections annually ranging from 50 to 1000 people in south-west India and the lack of effective treatments, a better understanding of this virus is needed. The development of a Reverse Genetics System for KFDV would provide the opportunity to address these issues in future studies. Using molecular techniques, the KFDV genome sequence was elucidated and the reverse genetics system was created. Utilizing this system live, infectious KFDV particles were produced from mammalian cell culture, thereby validating the success of the reverse genetics system. The implementation of this system will enable researchers to better study pathogenesis and disease progression, virus-host interplay, virion structure, genome replication and the emergence of effective therapeutics and vaccines.en
dc.description.noteMay 2010en
dc.format.extent1652338 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/1993/3962
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectKyasanur Forest Disease Virusen
dc.subjectReverse Geneticsen
dc.titleThe Molecular Characterization and The Generation of a Reverse Genetics System for Kyasanur Forest Disease Virusen
dc.typemaster thesisen_US
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