Identification and characterization of new cellular interacting proteins of HIV-1 integrase
| dc.contributor.author | Parvez, Md. Kamal Uddin | |
| dc.contributor.examiningcommittee | Fowke, Keith (Medical Microbiology) Peng, Zhikang (Immunology) Cheng, keding (Human Anatomy & Cell Science) | en |
| dc.contributor.supervisor | Yao, Xiaojian (Medical Microbiology) | en |
| dc.date.accessioned | 2011-04-12T17:31:06Z | |
| dc.date.available | 2011-04-12T17:31:06Z | |
| dc.date.issued | 2011-04-12T17:31:06Z | |
| dc.degree.discipline | Medical Microbiology | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | HIV-1 integrase (IN) enzyme employs several viral and cellular proteins for nuclear translocation and crucial integration of viral cDNA. Successful identification of new viral/cellular interactions may shed light for better understanding of HIV-1 replication. 293T cells were transiently transfected with pYEF-1-TAP-IN and cell lysate were subjected to Tandem Affinity Purification system to pull down putative IN-interacting cellular partners. A number of distinct bands from the Coomassie-stained gel were excised followed by in-gel digestion and mass spectrometry. Putative cellular partners of HIV-1 IN were heat shock protein 60 (HSP60), β-tubulin, γ-actin, ATP synthase alpha subunit and histone H1.2 were identified by mass spectrometry. Additionally, SF3A3 (splicing factor 3A3), another previously reported factor, was successfully co-immunoprecipitated with IN. The C-terminal portion of IN was found to be the region of interaction with SF3A3. Overall, this study has provided better understanding of IN dynamics enriching existing knowledge of HIV-1 IN biology. | en |
| dc.description.note | May 2011 | en |
| dc.format.extent | 7332987 bytes | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.uri | http://hdl.handle.net/1993/4525 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | HIV-1 | en |
| dc.subject | Integrase | en |
| dc.title | Identification and characterization of new cellular interacting proteins of HIV-1 integrase | en |
| dc.type | master thesis | en_US |